Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods

David Chenoweth is at University of Pennsylvania, PA, USA

• Enzyme- or light-triggered cyclopropenes for bioorthogonal ligationTing Jiang and Scott T. LaughlinAnalysis of metabolically labeled inositol phosphate messengers by NMRRobert Puschmann, Robert K. Harmel and Dorothea FiedlerPhoto-releasable derivatives of inositol pyrophosphatesSebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten SchultzIMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylationTimothy W. Bumpus, Dongjun Liang and Jeremy M. BaskinFluorescent bioorthogonal labeling of class B GPCRs in live cellsSrikanth Kumar Gangam and Qing LinNear-infrared photoactivatable nitric oxide donors with photoacoustic readout Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson ChanH2S donors with optical responsesMichael D. Pluth, Yu Zhao and Matthew M. CerdaVisualization of oxidative stress-induced carbonylation in live mammalian cellsKamalika Mukherjee, Tak Ian Chio and Susan L. BaneReceptor-based fluorescent sensors constructed from ribonucleopeptideSkun Nakano, Hiroaki Konishi and Takashi MoriiConformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopySiddharth S. Matikonda, Ralph Götz, Ryan McLaughlin, Markus Sauer and Martin J. SchnermannA near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophoreMichael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. SchnermannQuantitative measurement of cytosolic penetration using the chloroalkane penetration assayKirsten Deprey and Joshua A. KritzerCell-penetrating and mitochondrion-targeting moleculesGeorge Appiah Kubi and Dehua PeiA photocaged DNA nanocapsule for delivery and manipulation in cellsYihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki EndoA multicolor riboswitch-based platform for imaging of RNA in live mammalian cellsEsther Braselmann and Amy E. PalmerEnzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)Kayla N. Busby and Neal K. DevarajAssaying RNA solvent accessibility in living cells with LASERChao Feng and Robert C. SpitaleSplit T7 RNA polymerase biosensors to study multiprotein interaction dynamicsJeffrey A. Dewey and Bryan C. DickinsonCross-linking cellular nucleic acids via a target-directing double click reagentMasayuki Tera and Nathan W. LuedtkeClick chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probesSepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. RouhanifardQuantification of protein delivery in live cells using fluorescence correlation spectroscopy Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz