Characterization of Nanoparticles Intended for Drug Delivery

Inbunden, Engelska, 2017

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This second edition volume expands on the first edition by providing up-to-date protocols to characterize nanomaterials used as drug delivery agents.

Produktinformation

Utgivningsdatum2017-10-20
Mått178 x 254 x 21 mm
Vikt707 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor256
Upplaga2
FörlagHumana Press Inc.
ISBN9781493973507

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Evaluating Nanomedicines: Obstacles and Advancements.- Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test.- Considerations and Some Practical Solutions to Overcome Nanoparticle Interference with LAL Assays and to Avoid Endotoxin Contamination in Nanoformulations.- Elemental Analysis in Biological Matrices Using ICP-MS.- PEG Quantitation Using Reversed Phase High Performance Liquid Chromatography and Charged Aerosol Detection.- Quantitation of Surface Coating on Nanoparticles Using Thermogravimetric Analysis.- Immunoelectron Microscopy for Visualization of Nanoparticles.- Imaging of Liposomes by Transmission Electron Microscopy.- Updated Method for In Vitro Analysis of Nanoparticle Hemolytic Properties.- In Vitro Assessment of Nanoparticle Effects on Blood Coagulation.- In Vitro Analysis of Nanoparticle Effects on the Zymosan Uptake by Phagocytic Cells.- Assessing NLRP3 Inflammasome Activation by Nanoparticles.- Analysis of Complement Activation by Nanoparticles.- Methods for Analysis of Nanoparticle Immunosuppressive Properties In Vitro and In Vivo.- Analysis of Pro-Inflammatory Cytokine and Type II Interferon Induction by Nanoparticles.- Analysis of Nanoparticle Adjuvant Properties In Vivo.- In Vitro and In Vivo Methods for Analysis of Nanoparticle Potential to Induce Delayed Type Hypersensitivity Reactions.- Autophagy Monitoring Assay II: Imaging Autophagy Induction in LLC-PK1 Cells Using GFP-LC3 Protein Fusion Construct.- Improved Ultrafiltration Method to Measure Drug Release from Nanomedicines Utilizing a Stable Isotope Tracer.- Designing an In Vivo Efficacy Study of Nanomedicines for Preclinical Tumor Growth Inhibition.