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This new volume of Methods in Cell Biology looks at methods for analyzing centrosomes and centrioles. Chapters cover such topics as methods to analyze centrosomes, centriole biogenesis and function in multi-ciliated cells, laser manipulation of centrosomes or CLEM, analysis of centrosomes in human cancers and tissues, proximity interaction techniques to study centrosomes, and genome engineering for creating conditional alleles in human cells.
Covers sections on model systems and functional studies, imaging-based approaches and emerging studies
1. Correlative Light and Electron Microscopy Analysis of the Centrosome; A Step by Step Protocol.Dong Kong and Jadranka Loncarek2.Generation of a Conditional Analog-Sensitive Kinase in Human Cells Using CRISPR/Cas9-Mediated Genome EngineeringTyler Moyer and Andrew J. Holland3. Studying Centrosome Function Using Three-Dimensional Cell CulturesTeresa Arnandis and Susana A. Godinho4. Analysis of Centrosomes in Human CancerStefan Duensing5. In Vivo Examination of the Cortical Cytoskeleton in Multiciliated Cells Using Electron TomographyDaniel K. Clare, Maud Dumoux and Delphine Delacour6.Tackling Centrosome Biology Through Gene Targeting in Chicken B CellsPavithra L. Chavali and Fanni Gergely7. Centriole Biogenesis and Function in Multi-Ciliated CellsSiwei Zhang and Brian J. Mitchell8. Sub-Diffraction Resolution Microscopy Methods for Analyzing Centrosomes OrganizationVito Mennella, Rachel Hanna and Moshe Kim9. Probing Mammalian Centrosome Structure Using Bioid Proximity-Dependent BiotinylationElif Nur Firat-Karalar and Tim Stearns10. Purification of Centrosomes from Mammalian Cell LinesDelphine Gogendeau, Paul Guichard and Anne-Marie Tassin11. Isolation, Cryo-Tomography and Three-Dimensional Reconstruction of CentriolesPaul Guichard, Virginie Hamel, Aitana Neves and Pierre Gönczy12. New Insights into Centrosome Imaging in Drosophila and Mouse Neuroepithelial TissuesMaria A. Rujano, Renata Basto and Véronique Marthiens 13. Microinjection Techniques for Studying Centrosome Function in Drosophila Melanogaster Syncytial EmbryosPaul T. Conduit, Daniel Hayward and James G. Wakefield14. A Yeast Two-Hybrid Approach For Probing Protein-Protein Interactions at the CentrosomeBrian J. Galletta1 and Nasser M. Rusan15. Genome-Wide RNAi Screens in S2 Cells To Identify Centrosome ComponentsJeroen Dobbelaere16. Time- Lapse Recording of Centrosomes and Other Organelles in Drosophila NeuroblastsJudit Pampalona, Jens Januschke, Paula Sampaio, and Cayetano Gonzalez17. Centrosomes and Spindles in Ascidian Embryos and EggsAlex McDougall, Janet Chenevert, Gerard Pruliere, Vlad Costache, Celine Hebras, Gregory Salez and Remi Dumollard18. Ultrastructural Analysis of C. elegans CiliaDaniel Serwas and Alexander Dammermann19. Method: In Vitro Analysis of Pericentriolar Material AssemblyJeffrey B. Woodruff and Anthony A. Hyman20. Monitoring SPB Biogenesis in Fission Yeast with High Resolution and Quantitative Fluorescent MicroscopyImène B. Bouhlel, Kathleen Scheffler, Phong T. Tran and Anne Paoletti
Praise for the Series: "The series is invaluable for workers at all levels of cell biology." --Nature
