Celiac Disease

Methods and Protocols

Inbunden, Engelska, 2000

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Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world, present a collection of the state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both the novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays. Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

Produktinformation

Utgivningsdatum2000-04-01
Mått152 x 229 x undefined mm
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Medicine
Antal sidor288
FörlagHumana Press Inc.
ISBN9780896036505

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Sjukdomar och rubbningar inom Medicin

Celiac Disease.- Genotyping Methodologies.- From Linkage to Genes.- Linkage and the Transmission Disequilibrium Test in Complex Traits.- Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats.- Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry.- Synthetic Peptide Libraries for T-Cell Epitope Identification.- Characterization of HLA-DQ-Specific Peptide-Binding Motifs.- Studies of Gliadin-Specific T-Cells in Celiac Disease.- Morphometric Analysis of Intestinal Mucosa.- Morphometry of Rectal Mucosa.- Organ Culture of Rectal Mucosa.- In Situ Hybridization.- Measurements of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease.- Immunohistochemistry in Research and Diagnosis of Celiac Disease.- Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease.- Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease.- Whole Gut Lavage Fluid Analysis.