Del 129 i serien Methods in Molecular Medicine

Cardiovascular Disease, Volume 2

Molecular Medicine

Häftad, Engelska, 2010

1 589 kr

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The two volumes of Cardiovascular Disease: Methods and Protocols provide comprehensive coverage of both basic and advanced approaches to the study and characterization of cardiovascular disease. In Volume 1: Genetics and Volume 2: Molecular Medicine, highly experienced cardiovascular researchers describe in detail the most important techniques in molecular medicine that are employed in genetic, molecular, cellular, structural, and physiological studies of cardiovascular disease. A total of 37 chapters cover cutting-edge methods including cytogenetic analyses, linkage programs for mapping chromosomal locations of disease genes, bioinformatics, as well as human genetics for identifying genes for both monogenic and common complex diseases. Other areas covered also include mouse genetics for identifying genes for complex disease traits, microarray (genechips) analysis, proteomics, cell biology, physiology, animal models of human disease, gene therapy, vascular biology, and stem cells. The theory and principles of each method are described in detail, followed by a thorough description of materials and equipment needed, and step-by-step protocols for successful execution of the method.A Notes section provides advice for potential problems, any modifications, and alternative methods. Comprehensive and timely, both volumes of Cardiovascular Disease: Methods and Protocols serve as a valuable resource book for active researchers looking to advance knowledge of the mechanisms, diagnoses, and treatments of cardiovascular disease.

Produktinformation

Utgivningsdatum2010-11-10
Mått152 x 229 x 21 mm
Vikt549 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Medicine
Antal sidor363
FörlagHumana Press Inc.
ISBN9781617378294

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Hjärt- och kärlsjukdomar inom Medicin

Microarray Analysis of Cardiovascular Diseases.- Proteomics With Two-Dimensional Gel Electrophoresis and Mass Spectrometry Analysis in Cardiovascular Research.- Developing and Evaluating Genomics- or Proteomics-Based Diagnostic Tests.- Animal Models for Disease.- Generation of Transgenic Mice for Cardiovascular Research.- Quantitative Assay for Mouse Atherosclerosis in the Aortic Root.- Animal Models for Heart Failure.- Animal Models for Hypertension/Blood Pressure Recording.- Animal Models for Cardiac Arrhythmias.- Optical Mapping of Shock-Induced Arrhythmogenesis in the Rabbit Heart With Healed Myocardial Infarction.- Methods for Studying Voltage-Gated Sodium Channels in Heterologous Expression Systems.- Laser-Induced Thrombosis in Zebrafish Larvae.- Methods for Isolation of Endothelial and Smooth Muscle Cells and In Vitro Proliferation Assays.- Applications of Adenoviral Vector-Mediated Gene Transfer in Cardiovascular Research.- Murine and HIV-Based Retroviral Vectors for In Vitro and In Vivo Gene Transfer.- Efficient Transfection of Primary Cells Relevant for Cardiovascular Research by nucleofection®.- Cell Adhesion and Migration Assays.- Apoptosis Assays.- Preparation of Protein Crystals for X-Ray Structural Study.- Structural Elucidation of Integrin ?IIb?3 Cytoplasmic Domain by Nuclear Magnetic Resonance Spectroscopy.- Applications of Electron Cryo-Microscopy to Cardiovascular Research.- Stem Cells in Cardiovascular Disease.

From the reviews: "Second volume ... in its 22 chapters describes methods for comprehension of disease mechanism at the molecular level. ... Each chapter contains detailed protocols needed for each practical step in course of experimentation so that it may serve as a guide even for beginners. ... intended for undergraduate and graduate students, postdoctoral fellows, clinicians and basic researchers, who are entering new areas of cardiovascular research and/or designing new experiments. Many protocols could be applied in research of another diseases as well." (Lubica Lacinova, General Physiology and Biophysics, January, 2007)