• Cardiac Gene Expression.- Microarray Analysis of Gene Expression in Murine Cardiac Graft Infiltrating Cells.- Expression Profiling Using Affymetrix GeneChip® Probe Arrays.- Serial Analysis of Gene Expression (SAGE).- Functional Genomics by cDNA Subtractive Hybridization.- Statistical Methods in Cardiac Gene Expression Profiling.- Cardiac Gene Regulation.- Measurement of Cardiac Gene Expression by Reverse Transcription Polymerase Chain Reaction (RT-PCR).- Quantitative (Real-Time) RT-PCR in Cardiovascular Research.- RNase Protection Assay for Quantifying Gene Expression Levels.- In Situ Hybridization.- Cardiac Gene Regulation.- Characterization of cis-Regulatory Elements and Transcription Factor Binding.- Mapping Transcriptional Start Sites and In Silico DNA Footprinting.- Characterization of Cardiac Gene Promoter Activity.- In Silico Assessment of Regulatory cis-Elements and Gene Regulation.- Comparative Genomics.- Developing Computational Resources in Cardiac Gene Expression.- Cardiac Single Nucleotide Polymorphisms.- In Silico Analysis of SNPs and Other High-Throughput Data.- Discovery and Identification of Sequence Polymorphisms and Mutations with MALDI-TOF MS.- Gene Overexpression and Targeting in the Myocardium.- Conditional Targeting.- Cardiomyocyte Preparation, Culture, and Gene Transfer.- Adeno-Associated Viral Vector-Delivered Hypoxia-Inducible Gene Expression in Ischemic Hearts.- Lentivirus-Mediated Gene Expression.

"...a timely, detailed and well edited methodss text which would be a valuable addition to any cardiovascular research laboratory." -Molecular Biotechnology