Capillary Electrophoresis of Proteins

Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu

• Principles and practice of capillary electrophoresis: basic concepts; brand broadening in capillary electrophoresis; capillary electrophoresis intrumentation; preparative capillary electrophoresis; capillary electrophoresis separation modes. Detection of proteins: absorbance detection; fluorescence detection; mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes; use of buffer additives; capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method; sample preconcentration techniques; applications. Capillary isoelectric focusing: sample preparation and injection; focusing; two-step CIEF; single-step CIEF; capillary selection; detection; optimizing CIEF analysis; capillary IEF as a micro-preparative technique; applications; future developments. Sieving separation: analysis of native proteins; analysis of SDS-protein complexes. Concluding remarks.

". . .well written and referenced. It is one of the most comprehensive texts currently available on the application of capillary electrophoresis to protein analysis. . ..a useful source of information for separation scientists and postgraduate students wishing to obtain an overall review of protein capillary electrophoresis separations."---European Journal of Pharmaceutics and Biopharmaceutics