Capillary Electrophoresis of Proteins

Inbunden, Engelska, 1998

Av Tim Wehr, Roberto Rodriguez-Diaz, Mingde Zhu, USA) Wehr, Tim (Bay Bioanalytic Labs, Richmond, California, USA) Rodriguez-Diaz, Roberto (Dynavax Technologies, Berkeley, California

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Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions.

Produktinformation

Utgivningsdatum1998-10-20
Mått152 x 229 x 21 mm
Vikt720 g
FormatInbunden
SpråkEngelska
SerieChromatographic Science Series
Antal sidor300
FörlagTaylor & Francis Inc
ISBN9780824702052

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Principles and practice of capillary electrophoresis: basic concepts; brand broadening in capillary electrophoresis; capillary electrophoresis intrumentation; preparative capillary electrophoresis; capillary electrophoresis separation modes. Detection of proteins: absorbance detection; fluorescence detection; mass spectrometry. Strategies for reducing protein-wall interactions: operations at pH extremes; use of buffer additives; capillary coatings and other surface modifications. Capillary zone electrophoresis: developing a CZE method; sample preconcentration techniques; applications. Capillary isoelectric focusing: sample preparation and injection; focusing; two-step CIEF; single-step CIEF; capillary selection; detection; optimizing CIEF analysis; capillary IEF as a micro-preparative technique; applications; future developments. Sieving separation: analysis of native proteins; analysis of SDS-protein complexes. Concluding remarks.