• Introduction to Brain Tumor Stem Cells.- Isolation and Culture of Glioblastoma Brain Tumor Stem Cells.- Establishment and Culture of Patient-Derived Primary Medulloblastoma Cell Lines.- Bioinformatic Strategies for the Genomic and Epigenomic Characterization of Brain Tumors.- Detecting Stem Cell Marker Expression Using the NanoString nCounter System.- Flow Cytometric Analysis of Brain Tumor Stem Cells.- In Vitro Self-Renewal Assays for Brain Tumor Stem Cells.- Differentiation of Brain Tumor Initiating Cells.- The Study of Brain Tumor Stem Cell Migration.- The Study of Brain Tumor Stem Cell Invasion.- Cell Cycle Dynamics in Glioma Cancer Stem Cells.- Embryonic Stem Cell Models of Human Brain Tumors.- Chromatin Immunoprecipitation (ChIP) Protocols for the Cancer and Developmental Biology Laboratory.- EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF.- Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells.- In Vitro Assays for Screening Small Molecules.- Drug Delivery in an Orthotopic Tumor Stem Cell-Based Model of Human Glioblastoma.- Engineering Inducible Knock-In Mice to Model Oncogenic Brain Tumor Mutations from Endogenous Loci.- In Vivo Murine Models of Brain Metastasis.- Cellular Magnetic Resonance Imaging for Tracking Metastatic Cancer Cells in the Brain.

“Sheila K. Singh and Chitra Venugopal have developed a concise, systematic, and practical book; an ideal read for brain cancer researchers. … this book is recommended for anyone who strives for proficiency in biophysiology of cancer stem cells and hopes to discover novel therapeutic and treatment strategies for patients affected by devastating CNS disease.” (Angela M. Bohnen, Neurosurgery, Vol. 85 (4), October, 2019)