Bone Research Protocols

• Primary Human Osteoblast Cultures.- Osteoblast Isolation from Murine Calvaria and Long Bones.- Rat Osteoblast Cultures.- Isolation of primary avian Osteocytes.- Isolation of Mouse Osteocytes using Cell Fractionation for Gene Expression Analysis.- Studying Osteocyte Function using the Cell Lines MLO-Y4 and MLO-A5.- Isolation, Differentiation and Characterisation of Human Bone Marrow Stem Cells in vitro and in vivo.- Rodent Osteoclast Cultures.- Isolation and Culture of Primary Chicken Osteoclasts.- ISOLATION AND PURIFICATION OF RABBIT OSTEOCLASTS.- Generation of Human Osteoclasts from Peripheral Blood.- Osteoclast Formation in Mouse Co-Cultures.- Rankl- Mediated Osteoclast Formation from Murine RAW 264.7 Cells .- Transfection of Osteoclasts and Osteoclast Precursors.- Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation.- Analysis of Transcriptional Regulation in Bone Cells.- Extraction of Nucleic Acids from Bone.- Analysis of Gene Expression in Bone by Quantitative RT/PCR .- Histomorphometry in Rodents.- Studying Gene Expression in Bone by in situ Hybridization.- Immunostaining of Skeletal Tissue.- Techniques for the Study of Apoptosis in Bone.- Transmission Electron Microscopy of Bone.- Scanning Electron Microscopy of Bone.- Fluorescence Imaging of Osteoclasts using Confocal Microscopy.- Live Imaging of Bone Cell and Organ Cultures.- Analysis of Bone Architecture in Rodents using Micro Computed Tomography.- Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents.- Quantitative X-ray Imaging of Rodent Bone by Faxitron.- Bioluminescence Imaging of Bone Metastasis in Rodent.- Fourier Transform Infrared Imaging of Bone.- Raman Microscopy of Bone.- The Calvarial Injection Assay.- Ovariectomy/Orchidectomy in Rodents.- Mechanical Properties of Bone ex vivo.- Mechanical Stimulation of Bone Cells using Fluid Flow.- using CELL and organ culture models TO analyse responses OF bone cellS to mechanical STIMULATION .In vivo Mechanical Loading.

From the reviews of the second edition:“This volume presents detailed modern experimental methods to study bone biology. For bone researchers.” (Pediatric Endocrinology Reviews (PER), Vol. 9 (3), March, 2012)