After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering Ikuro Abe is Professor of Natural Products Chemistry at Graduate School of Pharmaceutical Sciences, The University of Tokyo (since 2009). He received his B.S. (1984) and Ph.D. (1989) from The University of Tokyo. After two years postdoctoral research with Professor Guy Ourisson at the CNRS Institut de Chimie des Substances Naturelles, and mostly with Professor Michel Rohmer at the Ecole Nationale Supérieure de Chimie de Mulhouse in France (1989-1991), he moved to the USA to work with Professor Glenn D. Prestwich at the State University of New York at Stony Brook (1991-1996) and then at the University of Utah (1996-1998). His research interests mostly focus on exploring and engineering the natural products biosynthesis. He received several awards, including Prizes for Science and Technology by the MEXT Japan (2019) and Medal with Purple Ribbon, Cabinet Office, Government of Japan (2025).

• 1. Experimental approaches for studying heme-oxygenase-like diiron enzymes acting on tryptophanBradley Moore, Sanjoy Adak and Maria-Eirini Pandelia2. Heme-dependent enzymeKatherine Ryan3. Multinuclear ion-dependent oxidation enzymes (MNIOs)Wilfred van der Donk4. The ThDP-dependent enzymes in alpha-keto acids coupling reactionsMing Ma5. Methylation-triggered terpene cyclizationsJeroen Dickschat and Kexin Yang6. Discovery and Biochemical Characterization of Pseudo-Sugar Transfer EnzymesTaifo Mahmud, Takeshi Tsunoda and Shumpei Asamizu7. Depside Bond-Forming Reactions in Polyketide SynthasesYudai Matsuda8. Functional characterization of a Rieske oxygenase in aeruginosin biosynthesisRichiro Ushimaru9. Biocatalytic Synthesis of α-Hydrazino Acid Derivatives by Cupin MetalloenzymesKenichi Matsuda10. Larg NRPS/PKS enzymesHui-Ming Ge11. P450 enzymeShengying Li12. Terpene cyclaseHsiao-Ching Lin13. NAD Metabolizing EnzymeTakayoshi Awakawa14. F420-dependent reactions in biosynthesis of secondary metabolitesGhader Bashiri15. Structure, function, and engineering of pyridoxal-5'-phosphate dependent enzymesTakahiro Mori16. SwnH2 and SwnH1 from the swainosnine pathwayYang Hai, Wenhui Xi and Shubin Mou17. Incorporation of Sulfur Catalyzed by the Radical SAM Enzyme MybB during the Biosynthesis of MycobacidinHung-wen Liu, Houyuan Zhao and Junling Bu18. Nonheme Fe/2OG enzymesWei-chen Chang, Yaoyao Shen, Kuo Wang, Philp M. Palacios and Yisong Guo19. NRPS (FR900359 side chain assembly and transesterification)Max Crüsemann20. Non-ribosomal peptide synthetaseWen Liu, Yanan Du and Yijiao Xiong21. CAH-like plant enzymeRyan S. Nett and Jaime Martinez Grundman22. Rieske oxygenaseJennifer D. Bridwell-Rabb

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering Ikuro Abe is Professor of Natural Products Chemistry at Graduate School of Pharmaceutical Sciences, The University of Tokyo (since 2009). He received his B.S. (1984) and Ph.D. (1989) from The University of Tokyo. After two years postdoctoral research with Professor Guy Ourisson at the CNRS Institut de Chimie des Substances Naturelles, and mostly with Professor Michel Rohmer at the Ecole Nationale Supérieure de Chimie de Mulhouse in France (1989-1991), he moved to the USA to work with Professor Glenn D. Prestwich at the State University of New York at Stony Brook (1991-1996) and then at the University of Utah (1996-1998). His research interests mostly focus on exploring and engineering the natural products biosynthesis. He received several awards, including Prizes for Science and Technology by the MEXT Japan (2019) and Medal with Purple Ribbon, Cabinet Office, Government of Japan (2025).

• 1. Experimental approaches for studying heme-oxygenase-like diiron enzymes acting on tryptophanBradley Moore, Sanjoy Adak and Maria-Eirini Pandelia2. Heme-dependent enzymeKatherine Ryan3. Multinuclear ion-dependent oxidation enzymes (MNIOs)Wilfred van der Donk4. The ThDP-dependent enzymes in alpha-keto acids coupling reactionsMing Ma5. Methylation-triggered terpene cyclizationsJeroen Dickschat and Kexin Yang6. Discovery and Biochemical Characterization of Pseudo-Sugar Transfer EnzymesTaifo Mahmud, Takeshi Tsunoda and Shumpei Asamizu7. Depside Bond-Forming Reactions in Polyketide SynthasesYudai Matsuda8. Functional characterization of a Rieske oxygenase in aeruginosin biosynthesisRichiro Ushimaru9. Biocatalytic Synthesis of α-Hydrazino Acid Derivatives by Cupin MetalloenzymesKenichi Matsuda10. Larg NRPS/PKS enzymesHui-Ming Ge11. P450 enzymeShengying Li12. Terpene cyclaseHsiao-Ching Lin13. NAD Metabolizing EnzymeTakayoshi Awakawa14. F420-dependent reactions in biosynthesis of secondary metabolitesGhader Bashiri15. Structure, function, and engineering of pyridoxal-5'-phosphate dependent enzymesTakahiro Mori16. SwnH2 and SwnH1 from the swainosnine pathwayYang Hai, Wenhui Xi and Shubin Mou17. Incorporation of Sulfur Catalyzed by the Radical SAM Enzyme MybB during the Biosynthesis of MycobacidinHung-wen Liu, Houyuan Zhao and Junling Bu18. Nonheme Fe/2OG enzymesWei-chen Chang, Yaoyao Shen, Kuo Wang, Philp M. Palacios and Yisong Guo19. NRPS (FR900359 side chain assembly and transesterification)Max Crüsemann20. Non-ribosomal peptide synthetaseWen Liu, Yanan Du and Yijiao Xiong21. CAH-like plant enzymeRyan S. Nett and Jaime Martinez Grundman22. Rieske oxygenaseJennifer D. Bridwell-Rabb

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering Ikuro Abe is Professor of Natural Products Chemistry at Graduate School of Pharmaceutical Sciences, The University of Tokyo (since 2009). He received his B.S. (1984) and Ph.D. (1989) from The University of Tokyo. After two years postdoctoral research with Professor Guy Ourisson at the CNRS Institut de Chimie des Substances Naturelles, and mostly with Professor Michel Rohmer at the Ecole Nationale Supérieure de Chimie de Mulhouse in France (1989-1991), he moved to the USA to work with Professor Glenn D. Prestwich at the State University of New York at Stony Brook (1991-1996) and then at the University of Utah (1996-1998). His research interests mostly focus on exploring and engineering the natural products biosynthesis. He received several awards, including Prizes for Science and Technology by the MEXT Japan (2019) and Medal with Purple Ribbon, Cabinet Office, Government of Japan (2025).

• 1. Experimental approaches for studying heme-oxygenase-like diiron enzymes acting on tryptophanBradley Moore, Sanjoy Adak and Maria-Eirini Pandelia2. Heme-dependent enzymeKatherine Ryan3. Multinuclear ion-dependent oxidation enzymes (MNIOs)Wilfred van der Donk4. The ThDP-dependent enzymes in alpha-keto acids coupling reactionsMing Ma5. Methylation-triggered terpene cyclizationsJeroen Dickschat and Kexin Yang6. Discovery and Biochemical Characterization of Pseudo-Sugar Transfer EnzymesTaifo Mahmud, Takeshi Tsunoda and Shumpei Asamizu7. Depside Bond-Forming Reactions in Polyketide SynthasesYudai Matsuda8. Functional characterization of a Rieske oxygenase in aeruginosin biosynthesisRichiro Ushimaru9. Biocatalytic Synthesis of α-Hydrazino Acid Derivatives by Cupin MetalloenzymesKenichi Matsuda10. Larg NRPS/PKS enzymesHui-Ming Ge11. P450 enzymeShengying Li12. Terpene cyclaseHsiao-Ching Lin13. NAD Metabolizing EnzymeTakayoshi Awakawa14. F420-dependent reactions in biosynthesis of secondary metabolitesGhader Bashiri15. Structure, function, and engineering of pyridoxal-5'-phosphate dependent enzymesTakahiro Mori16. SwnH2 and SwnH1 from the swainosnine pathwayYang Hai, Wenhui Xi and Shubin Mou17. Incorporation of Sulfur Catalyzed by the Radical SAM Enzyme MybB during the Biosynthesis of MycobacidinHung-wen Liu, Houyuan Zhao and Junling Bu18. Nonheme Fe/2OG enzymesWei-chen Chang, Yaoyao Shen, Kuo Wang, Philp M. Palacios and Yisong Guo19. NRPS (FR900359 side chain assembly and transesterification)Max Crüsemann20. Non-ribosomal peptide synthetaseWen Liu, Yanan Du and Yijiao Xiong21. CAH-like plant enzymeRyan S. Nett and Jaime Martinez Grundman22. Rieske oxygenaseJennifer D. Bridwell-Rabb