This book constitutes the proceedings of the 14th International Conference on Biomimetic and Biohybrid Systems, Living Machines 2025, held in Sheffield, UK, during July 15-18, 2025.The 43 full papers and 7 short papers presented were carefully reviewed and selected from 67 submissions.The papers were organized in topical sections as follows: Bioinspired robotics and biohybrids; Soft robotics; Applications and machine learning; Cognitive and computational neuroscience; Navigation, sensors, and perception; Ethics and responsibility; Biomechanics and proprioception.