Biomarker Methods in Drug Discovery and Development

• Biomarker Sample Collection and Handling in the Clinical Setting to Support Early-Phase Drug Development.- Gene Expression-Based Biomarkers of Drug Safety.- Profiling Gene Expression Signatures Using Fluorescent Microspheres.- Real-Time Polymerase Chain Reaction Gene Expression Assays in Biomarker Discovery and Validation.- SAGE Analysis in Identifying Phenotype Single Nucleotide Polymorphisms.- Single Nucleotide Polymorphisms and DNA Methylation Analysis Using Pyrosequencing Methods.- The Application of Laser Capture Microdissection for the Analysis of Cell-Type-Specific Gene Expression in a Complex Tissue:.- The Use of Two-Dimensional Gel Electrophoresis for Plasma Biomarker Discovery.- Difference In-Gel Electrophoresis: A High-Resolution Protein Biomarker Research Tool.- Label-Free Mass Spectrometry-Based Protein Quantification Technologies in Protein Biomarker Discovery.- Top-Down Quantitative Proteomic Analysis Using a Highly Multiplexed Isobaric Mass Tagging Strategy.- SELDI Technology for Identification of Protein Biomarkers.- Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens.- LC-MS Metabonomics Methodology in Biomarker Discovery.- GC-MS-Based Metabolomics.- NMR-Based Metabolomics for Biomarker Discovery.- Unraveling Glycerophospholipidomes by Lipidomics.

From the reviews: "... extremely useful to pharmaceutical scientists who are involved in research all the way from early drug discovery through clinical trials." - Doody's Book Review "The book is ... for the bench scientist and is full of helpful advice on how to achieve high quality data using up-to-date technical approaches in genomics, proteomics and metabonomics. ... I warmly recommend this ... to all practicing toxicologists." (Roy Forster, British Toxicological Society Newsletter, June-August, 2009)