Thisbook constitutes the refereed proceedings of the 4th International Conferenceon Bioinformatics and Biomedical Engineering, IWBBIO 2016, held in Granada,Spain, in April 2016.The69 papers presented were carefully reviewed and selected from 286 submissions.The scope of the conference spans the following areas: bioinformatics for healthcareand diseases; biomedical image analysis; biomedical signal analysis;computational systems for modeling biological processes; eHealth; tools fornext generation sequencing data analysis; assistive technology for people withneuromotor disorders; fundamentals of biological dynamics and maximization ofthe information extraction from the experiments in the biological systems; highperformance computing in bioinformatics, computational biology andcomputational chemistry; human behavior monitoring, analysis and understanding;pattern recognition and machine learning in the -omics sciences; and resourcesfor bioinformatics.