Claire Prigent-Combaret is a CNRS scientist working on the ecological and molecular mechanisms of plant growth promotion and plant disease control by root-associated bacteria. She is the head of the Rhizosphere group in the Microbial Ecology Laboratory at University of Lyon 1, France.Bernard Dumas is a CNRS scientist working on plant immunity and microbial effectors. He is director of the Plant Science Laboratory (LRSV CNRS – Toulouse University, France) and coordinator of the BioPlantProducts laboratory, whose objective is to develop natural products for plant protection and stimulation.

• Introduction xiBernard DUMAS and Claire PRIGENT-COMBARETChapter 1 Regulatory Aspects of Biocontrol 1Diane ROBIN, Léa MERLET and Patrice MARCHAND1.1 Regulatory definition of biocontrol 21.2 Current issues and limitations 31.3 A mixed evolution 51.4 Necessary evolutions 121.5 Conclusion 131.6 References 14Chapter 2 Biological Controls in Horticulture 19Nicolas GUIBERT, Maïder SAINT JEAN, Claire PRIGENT-COMBARET, Jean-Marc DEOGRATIAS, Cécile CABASSON and Pierre PÉTRIACQ2.1 Introduction 192.2 Biological controls in horticulture 242.3 Physiological trade-offs for growth and immunity 262.4 Eco-innovations and economic trade-offs 282.5 Challenges and perspectives 362.6 Concluding remarks 382.7 References 39Chapter 3 Development of Omics Tools for the Assessments of the Environmental Fate and Impact of Biocontrol Agents 47Hikmat GHOSSON, Marie-Virginie SALVIA and Cédric BERTRAND3.1 Introduction: emergence of biocontrol agents and their risks 483.2 Evaluation methodologies: an overview 493.3 Limitations of classic methodologies 503.4 Omics: potential tools for risks assessment? 523.5 Perspectives 623.6 List of abbreviations 633.7 Acknowledgments 643.8 References 65Chapter 4 Plant Secondary Metabolites Mode of Action in the Control of Root-Knot Nematodes 75Nikoletta NTALLI and Pierluigi CABONI4.1 Introduction 764.2 Recent research on the use of plant secondary metabolites to control Meloidogyne spp 774.3 Conclusion 834.4 References 83Chapter 5 Agro-industrial By-products and Waste as Sources of Biopesticides 91Maria Fe ANDRES and Azucena GONZÁLEZ COLOMA5.1 Introduction 915.2 Biopesticidal properties of pyrolysis products from agro-industrial waste 935.3 Biopesticidal properties of hydrolates: by-products of essential oil distillation 995.4 Biopesticidal properties of olive oil mill waste 1035.5 Conclusion and future directives 1055.6 Acknowledgments 1075.7 References 107Chapter 6 Antimicrobial and Defense Elicitor Peptides as Biopesticides for Plant Disease Control 121Emilio MONTESINOS, Esther BADOSA, Maria PLA, Laura MONTESINOS and Anna BONATERRA6.1 Introduction 1226.2 Peptides of microbial origin 1246.3 Peptides from plants 1306.4 Peptides from animals 1346.5 Synthetic peptides 1386.6 Biotechnological production of peptides 1426.7 References 145Chapter 7 Biocontrol of Plant Pathogens via Quorum Quenching 159Denis FAURE and Xavier LATOUR7.1 Quorum quenching to counteract quorum sensing 1597.2 Quorum sensing inhibitors 1607.3 Quorum quenching enzymes 1617.4 Quorum quenching biocontrol agents 1627.5 Monitoring of quorum quenching biocontrol agents and activities 1637.6 Biostimulation of quorum quenching 1647.7 Management of quorum quenching treatments 1657.8 Quorum quenching in biocontrol: perspectives 1667.9 Acknowledgments 1667.10 References 167Chapter 8 Phage-mediated Biocontrol Against Plant Pathogenic Bacteria 173Fernando CLAVIJO-COPPENS, Clara TORRES-BARCELO, Mireille ANSALDI, Nicolas TAVEAU and Denis COSTECHAREYRE8.1 Introduction 1748.2 Bacteriophages for plant health 1798.3 Phage-based biocontrol regulations 1998.4 Conclusions and perspectives 2018.5 Acknowledgments 2038.6 References 203Chapter 9 Microbiome-assisted Agriculture: Current Knowledge and Future Directions 217Ke YU, Hongwei LIU, Wei ZHONG and Ioannis A STRINGLIS9.1 Introduction 2179.2 Microbiome-mediated benefits for plants 2219.3 Chemical cues derived from plants and microbes guide microbiome assembly 2259.4 Plant and soil microbiome engineering 2329.5 Concluding remarks and future perspectives 2389.6 References 239List of Authors 255Index 259