Biocatalysis for Green Chemistry and Chemical Process Development

JUNHUA (Alex) TAO is currently CEO of Metabomics, Inc. Prior to that; he was the CSO of Elevance Renewable Sciences, and the creator and head of the Biotransformations Group at Pfizer, where he was also a core member of the API Development Team. He co-authored over 60 publications including “Biocatalysis for the Pharmaceutical Industry” (Wiley, 2009) and co-invented over 20 patents. In addition, he was the recipient of the Pfizer Achievement Award, the Pfizer Green Chemistry Award, and a key member of the Lyrica  process development team, which won the IChemE Award for Excellence in Green Chemistry and Engineering (2006). ROMAS JOSEPH KAZLAUSKAS is Professor of Biochemistry, Molecular Biology and Biophysics at the BioTechnology Institute of the University of Minnesota, Twin Cities. He is the coauthor of over eighty papers and reviews, and the book Hydrolases in Organic Synthesis. He also served as the co-chair of two Gordon Research Conferences, one on biocatalysis and one on green chemistry.

• Contributors vii Preface xiPart I. Introduction Chapters 11. Biotechnology Tools for Green Synthesis: Enzymes, Metabolic Pathways, and Their Improvement by Engineering 32. How Green Can the Industry Become with Biotechnology? 233. Emerging Enzymes and Their Synthetic Applications 454. Reaction Efficiencies and Green Chemistry Metrics of Biotransformations 67Part II. Application and Case Studies-Pharmaceuticals and Fine Chemicals 895. Biocatalytic Routes to Chiral Intermediates for Development of Drugs 916. Transglutaminase for Protein Drug Modification: Pegylation and Beyond 1517. Microbial Production of Plant-Derived Pharmaceutical Natural Products Through Metabolic Engineering: Artemisinin and Beyond 1738. Toward Greener Therapeutic Proteins 197Part III. Application and Case Studies-Flavor & Fragrance, Agrochemicals and Fine Chemicals 2219. Opportunities for Biocatalysis in the Flavor, Fragrance, and Cosmetic Industry 22310. Application of Biocatalysis in the Agrochemical Industry 25511. Green Porduction of Fine Chemicals by Isolated Enzymes 27712. Whole Cell Production of Fine Chemicals and Intermediates 299Part IV. Application and Case Studies-Polymers and Renewable Chemicals 32713. Green Chemistry for the Production of Biodegradable, Biorenewable, Biocompatible, and Polymers 32914. Enzymatic Degradation of Lignocellulosic Biomass 36115. Bioconversion of Nenewables-Plant Oils 39116. Microbial Bioprocesses for industrial-Scale Chemical Production 429Index 469

“Therefore, this book is a valuable compendium for researchers and users of biocatalysis from both, the academic and the industrial world.”  (Catal Lett, 1 March 2013)“This book is well organised, containing most key subjects one would expect in the multiple research areas of biocatalysis. It is suitable for graduate level students, postdocs, and industrial researchers. It also can be regarded as an auxiliary textbook for students who wish to know the pros and cons of biocatalysis in chemical processes, and what the future holds for the development of biotechnology in green chemistry.”  (Chemistry World, 2012)