Bifidobacteria

Methods and Protocols

Inbunden, Engelska, 2021

Av Douwe van Sinderen, Marco Ventura, Douwe Van Sinderen, Douwe van Sinderen

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Chapters guide readers through experimental protocols on procedures to isolate and cultivate bifidobacteria, taxonomic identification of bifidobacterial isolates, sequencing and annotate genomes, physiologically characterize bifidobacteria, and methods on the genetic manipulation of bifidobacterial strains.

Produktinformation

Utgivningsdatum2021-03-03
Mått178 x 254 x 20 mm
Vikt678 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor239
FörlagSpringer-Verlag New York Inc.
ISBN9781071612736

Tillhör följande kategorier

Methods for the Isolation and Recovery of Bifidobacteria.- Bifidobacterium Transformation.- Isolation of Chromosomal and Plasmid DNA from Bifidobacteria.- Assembly, Annotation, and Comparative Analysis of Bifidobacterial Genomes.- Site-directed Mutagenesis of Bifidobacterium Strains.- Protocol to Select Bifidobacteria from Fecal and Environmental Samples.- Phageome Analysis of Bifidobacteria-Rich Samples.- Measuring Conjugated Linoleic Acid (CLA) Production by Bifidobacteria.- Detection, Isolation, and Purification of Bifidobacterial Exopolysaccharides.- Determination of Bifidobacterial Carbohydrate Utilisation Abilities and Associated Metabolic End-Products.- Model for Murine Gut Colonisation by Bifidobacteria.- Identification of Bifidobacteria by the Phosphoketolase Assay.- Determination of Bile Salt Hydrolase Activity in Bifidobacteria.- A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications.- Metagenomic Analyses of Bifidobacterial Communities.- Resistance of Bifidobacteria toward Antibiotics.- In vitro Assessment of Prebiotic Activity.- Bifidobacterium Genome Assembly and Methylome Analysis using Pacbio SMRT Sequencing.