Basic Life Science Methods

Dr. Javeed Ahmad Tantray’s current research focus is on Human cardiovascular diseases obtained his Ph. D from Osmania University in Hyderabad, India. Dr. Tantray has done Post Doc studies with the Department of Zoology, University of Kashmir, receiving funding from the UGC-CSIR. He published many National and International peer-reviewed research articles. Dr. Tantray has published in high impact factor journals such as the International Journal of Cardiology, and Saudi journal of biological science. He has guided 50, M.Sc / and graduate students projects. He has received Junior scientist awards both at national and international level. He received the Best Abstract Award at the 4th Asia Pacific CardioMetabolic Syndrome Congress held by the Korean Society of CardioMetabolic Syndrome,with an award amount of USD 500. Dr. Tantray has organized a national seminar on "Cancer Bio-markers" and a one-day national workshop on “Assembly and usage of Foldscope”. Dr. Tantray is currently an Assistant Professor in the Department of Zoology, School of life sciences central university of Kashmir (J&K). Nighat Un Nissa has completed her M.Sc. and M.Phil. From Department of zoology, University of Kashmir. Ms. Nissa is currently pursuing her Ph.D. in Zoology and is working on Fish parasites and their impact on pollution. She has published her research work in National and International journals. She was awarded Gold Medal for poster presentation in Animal science congress held by Department of Zoology, University of Kashmir. Rasy Fayaz Choh Wani PhD has completed her M.Sc. degree from Kashmir University. Ms. Rasy is currently pursuing Ph.D. She has published her research work in many reputed journals. She has presented her different papers in different international and national conferences. She is currently on research topic “Isolation and characterization of pathogenic bacterial microflora associated with cultured Cyprinus carpio in Kashmir Valley”. Dr. Sheikh Mansoor Shafi currently serves as a postdoctoral researcher at the Phenomics Laboratory within Jeju National University in South Korea. His primary research focuses on high-throughput phenomics, remote sensing and precision agriculture. Prior to this role, he held positions as a Postdoc Research Associate at SKIMS Srinagar and as a Project Associate-I at CSIR-Indian Institute of Integrative Medicine Jammu (IIIM). Throughout his academic journey, Dr. Mansoor has had the opportunity to work in reputed laboratories, including the Transcriptomics Labs at SKUAST Kashmir and the Department of Biotechnology and Pathology at Central Institute of Temperate Horticulture (CITH) Rangreth Srinagar. His educational background comprises a BSc degree from SP College of Science (University of Kashmir), an MSc degree from HNBGU Uttarakhand, and a Ph.D. degree from Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu.

• 1. Calculations of Molarity and Normality2. Glassware washing and sterilization techniques3. pH Meter: Its use and calibration4. Principle and working of Autoclave5. Principle and working of Centrifuge6. Principles and working of Laminar7. Estimation of Total cholesterol (Flegg 1972)8. Estimation of Creatinine9. Estimation of blood Urea10. Estimation of blood glucose By GOD – POD method11. Determination of Albumin, and Globulin in Plasma12. Quantitative determination of Homocysteine13. Estimation of serum bilirubin14. Estimation of diagnostic enzyme ALT15. Estimation of diagnostic enzyme AST16. To Estimate Protein by Lowry’s Method17. Estimation of Reducing sugar by using Dinitro Salicylic Acid (DNS) Method18. To perform quantitative determination of total protein in Serum/plasma19. Enzyme Activity Using Starch assay20. To Estimate Protein by Bradford’s Assay21. To Identify a Protein Sample Using SDS-PAGE22. Genomic DNA isolation from whole blood23. PCR for gene amplification24. Agarose Gel Electrophoresis (AGE)25. RNA Isolation from Human tissue26. Real-time polymerase chain reaction (RT-qPCR)27. Preparation of competent cells by CaCl2 treatment28. RNA Isolation from Plant tissues29. DNA isolation from the bacterial culture broth30. DNA isolation from fungal Mycelium31. Quantification of the DNA using spectrophotometer32. Formaldehyde gel electrophoresis of the isolated RNA sample33. Paper chromatography of different amino acid34. Separation of plants pigments using Column Chromatography35. Separation of plant pigment by Thin Layer Chromatography36. Chromatin Immunoprecipitation (ChIP) Protocol37. Types of Media38. Preparation of Nutrient Agar Media39. Serial Dilution of the Bacterial Cells40. Spread Plate Method of The Bacterial Cells41. Streak Plate Method42. Identified Bacterial Colony in Culture Broth43. Pour plate method for bacterial colony counting44. Gram Staining of Bacteria45. Casein hydrolysis test46. Antibiotic resistance test by Agar Well Diffusion Method