• Acknowledgments How To Use This Manual PART I: AN OVERVIEW OF BACULOVIRUSES 1: Virus Structure and the Infection Process 2: Gene Organization, Regulation, and Function 3: Virus-Host Interactions 4: Summary of Baculovirus Features Relevant to Expression Factors PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS 5: Choice of Virus and Host Species 6: Choice of Transfer Plasmid 7: Available Transfer Plasmids 8: Choosing a Parent Virus for Use in Vector Construction 9: Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION 10: Safety Considerations 11: Insect Cell Culture 12: Virus Methods 13: Contransfection and Recombinant Virus Identification 14: Characterizing Recombinant Gene Expression 15: Post-Translational Modification PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE 16: David L. Clemm: Scale-Up of Protein Production in a Stirred Bioreactor 17: Cheryl Isaac Murphy: Scale-Up of Protein Production in an Airlift Fermenter 18: Insect Rearing and Infection Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region Appendix 2: Nucleotide Sequence of AcMNPV p10 Region Appendix 3: List of Genes Expressed in the Baculovirus Expression System Appendix 4: Suppliers Appendix 5: Stock Solutions Appendix 6: Excel Spreadsheet for TCID[5[0 Calculation References Index

"A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, etal. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work." --Cell"Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overviewprior to taking the plunge." --Tibtech"A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, etal. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work." --Cell"Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overviewprior to taking the plunge." --Tibtech