B Cell Protocols

Inbunden, Engelska, 2004

AvHua Gu,Klaus Rajewsky,Hua Gu,Klaus Rajewsky

1 999 kr

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A diverse collection of protocols ranging from the latest inventions and applications to some classic, but still frequently used, methods for studying B-lymphocyte development and function in higher animals. These readily reproducible protocols follow the successful Methods in Molecular Biology series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Produktinformation

Utgivningsdatum2004-05-12
Mått155 x 235 x 24 mm
Vikt679 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor292
Upplaga2004
FörlagHumana Press Inc.
ISBN9781588293473

Tillhör följande kategorier

Cellbiologi inom Naturvetenskap och teknik

Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen.- Characterization of B-Cell Maturation in the Peripheral Immune System.- Identification of B-Cell Subsets.- Analysis of B-Cell Life-Span and Homeostasis.- Differentiation of B Lymphocytes From Hematopoietic Stem Cells.- Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System.- Conditional Gene Mutagenesis in B-Lineage Cells.- Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells.- T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences.- of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors.- Immunoglobulin Class Switching.- In Vitro and In Vivo Assays of B-Lymphocyte Migration.- Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo.- B-Cell Signal Transduction.- Isolation of Lipid Rafts From B Lymphocytes.- Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells.- Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice.- Analysis of B-Cell Signaling Using DT40 B-Cell Line.- B-Cell Development and Pre-B-1 Cell Plasticity In Vitro.