Avidin-Biotin Interactions

• Preparation of Avidin Conjugates.- Coupling of Antibodies With Biotin.- Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin.- Use of the Avidin (Imino)Biotin System as a General Approach to Affinity Precipitation.- Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis.- Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS–PAGE Using Biotin-4-Fluorescein.- Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives.- Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based Affinity Chromatography Resins.- High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli.- Endogenous Biotin in Rat Brain.- Pseudo-Immunolabelling With the Avidin–Biotin–Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina.- Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones.- Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay.- Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin.- Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure.- A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin.- Biotin–Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications.

Aus den Rezensionen: "... Das ... editierte Methodenbuch gliedert sich in siebzehn Beitrage, die von auf dem jeweiligen Gebiet ausgewiesenen Autoren verfasst wurden. ... Die angegebenen Protokolle ermoglichen, einem Methodenbuch entsprechend, eine zeitsparende und kostengunstige Praparation ... Positiv ist auch die gute Darstellung der Faktoren, die fur Biotinylierungsreaktionen ... kritisch sind. ... Das Buch ist nach Form und Inhalt in jedem Fall geeignet, dem Leser die weitere Vertiefung in die aktuelle Literatur zu ermoglichen und Hinweise fur Applikationen zu geben. ... eine umfassende Informationsquelle und Protokolle zur Verfugung ..." (Dr. rer. nat. H. Walzel, in: Laboratoriums-Medizin: journal of laboratory medicine, 2009, Vol.33, Issue 2, S. 129 f.)