This truly unique volume provides a broad spectrum of techniques and assays pertinent for experimental atherosclerosis research. Practical information regarding sample collection, choice of model system, experimental design, and data analysis techniques are provided in each chapter. In addition novel animal models are discussed including genetically engineered mice. Traditional and newer methods for identifying and separating classes and subclasses of lipoproteins are included along with assays for more recently identified plasma proteins implicated in atherogenesis, such as cholesteryl ester transfer protein, homocysteine, glycated lipoproteins, and apolipoprotein. Chapters describe the isolation and culture of cells and clycosaminoglycans from atherosclerotic plaques and further elaborate on in vivo techniques for the collection and analysis of experimental atherosclerotic lesions. Finally, a comprehensive presentation of gene therapies is given, and is accompanied by a highly practical technique that has been successfully implemented in pigs.The compilations in this manual will benefit those researchers in pursuit of understanding, diagnosing, treating, and ultimately preventing atherosclerosis.

Produktinformation

Utgivningsdatum2000-10-31
Mått155 x 235 x undefined mm
Vikt540 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Medicine
Antal sidor247
Upplaga2001
FörlagHumana Press Inc.
ISBN9780896037519

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Hjärt- och kärlsjukdomar inom Medicin
Naturvetenskap:allmänt inom Naturvetenskap och teknik

Animal Models of Diet-Induced Atherosclerosis.- Mechanical Injury Models.- Genetically Manipulated Models of Atherosclerosis in Mice.- Lipoprotein Isolation and Analysis from Serum by Preparative Ultracentrifugation.- Separation of Plasma Lipoproteins in Self-Generated Gradients of Iodixanol.- Fractionation of Lipoprotein Subclasses in Self-Generated Gradients of Iodixanol.- 7 Detecting Cholesteryl Ester Transfer Protein in Plasma.- 8 Determination of Plasma Homocysteine.- 9 Assay for Serum Glycated Lipoproteins.- 10 Lipoprotein(a) Quantitation.- Isolation and Characterization of Glycosaminoglycans from Human Atheromatous Vessels.- Culture of Human Smooth Muscle Cells.- Culture of Human Endothelial Cells.- Assay for Cell Migration and Invasion of Vascular Smooth Muscle Cells.- Collection and Processing of Arterial Specimens for Histological Analysis.- Intravascular Ultrasound Imaging in the Quantitation of Atherosclerosis in Vivo.- Identification of Cell Types and Quantification of Lesion Composition.- 1Nonradioactive In Situ Hybridization in Atherosclerotic Tissue.- Evaluation of Angiogenesis.- Evaluation of Cell Proliferation in Human Atherosclerotic Lesions.- Gene Transfer to the Vasculature Historical Perspective and Implications for Future Research Objectives.- A Pig Model of Vein Graft Disease Applications for Potential Gene Therapies.

"Methods for setting up animal model periments and special assays of blood and cellular moments of the pathologic processes involved are thoroughly described and referenced. Newly emerging animal models based on genetic manipulation are described in depth, with sufficient detail to broaden the scope of research by seasoned investigators to permit the novice to enter this field. ...Anyone working in vascular pathology and atherosclerosis research will find a wealth of practical information describing modern research methods." - Clinical and Applied Thrombosis/Hemostasis "Atherosclerosis is recommended for academic health science libraries. It is also recommended to individual laboratories that conduct research in the area of atherosclerosis or heart disease." -E-STREAMS