This detailed volume gathers methods essential for investigating the multifaceted roles of astrocytes in both physiology and pathological conditions. The book covers several techniques for the preparation of astrocytes from animal models, the examination of astrocyte morphology and functions in vitro and ex vivo, as well as the investigation of astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Astrocytes: Methods and Protocols, Second Edition serves as an ideal guide for both experienced and beginner scientists working toward unravelling the roles of these highly versatile cells.