The advancement of artificial seed technology in the field of plant tissue culture has been met with great enthusiasm and rapid growth. At present, this method is recognized as an effective substitute for plant propagation of commercially significant plant materials.Furthermore, this technology has proven to be a valuable means of handling cells and tissues, safeguarding them from external gradients, and facilitating short-term and long-term storage under low and ultra-low temperature conditions, respectively. Moreover, it serves as an efficient delivery system.The information regarding synthetic seed preparation technology, including its implications, achievements, and limitations, is currently scattered across various journal articles and edited books. However, this book presents a comprehensive overview of the subject, with up-to-date citations, providing a comprehensive review of the recent advancements in the field.