This book constitutes the thoroughly refereed workshop post-proceedings of the 16th International Workshop on Approximation and Online Algorithms, WAOA 2018, held in Helsinki, Finland, in August 2018 as part of ALGO 2018.The 19 revised full papers presented together with one invited paper in this book were carefully reviewed and selected from 44 submissions. Topics of interest for WAOA 2016 were: graph algorithms; inapproximability results; network design; packing and covering; paradigms for the design and analysis of approximation and online algorithms; parameterized complexity; scheduling problems; algorithmic game theory; algorithmic trading; coloring and partitioning; competitive analysis; computational advertising; computational finance; cuts and connectivity; geometric problems; mechanism design; resource augmentation; and real-world applications.