This book constitutes the revised selected papers of the 5th International Conference on Applied Intelligence and Informatics, AII 2025, held in Washington, DC, USA, during October 28–30, 2025.The 41 full papers included in these proceedings were carefully reviewed and selected from 127 submissions. They were organized in the following topical sections: Emerging Applications of AI and Informatics; Application of AI and Informatics in Healthcare; Application of AI and Informatics in Pattern Recognition; and Application of AI and Informatics in Network, Security and Analytics.