This book constitutes thoroughly refereed and revised selected papers from the 12th Iberoamerican Conference on Applications and Usability of Interactive TV, jAUTI 2023, held in Havana, Cuba, during October 16–17, 2023.The 9 full papers included in this book were carefully reviewed and selected from 25 submissions. They were organized in topical sections as follows: ​technologies, services and content creation for interactive digital TV; health and socialization through interactive TV; user interfaces, interaction and accessibility.