Antigen Processing

Methods and Protocols

Inbunden, Engelska, 2019

AvPeter van Endert,Peter Van Endert,Peter van Endert

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The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules.

Produktinformation

Utgivningsdatum2019-05-30
Mått178 x 254 x 31 mm
Vikt1 085 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor458
Upplaga2
FörlagHumana Press Inc.
ISBN9781493994496

Tillhör följande kategorier

Onkologi inom Medicin
Medicinsk utrustning och medicinska tekniker inom Medicin

Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes.- Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry.- Trimming of MHC Class I Ligands by ERAP Aminopeptidases.- Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases.- Testing the Impact of Protease Inhibitors in Antigen Presentation Assays.- In Vitro Studies of MHC Class I Peptide Loading and Exchange.- Measuring Synthesis and Degradation of MHC Class I Molecules.- Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules.- A SIINFEKL Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics.- Purification and Identification of Naturally Presented MHC Class I and II Ligands.- Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs.- Quantitating MHC Class I Ligand Production and Presentation using TCR-LikeAntibodies.- Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors.- A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway.- Evaluating CD8+ T Cell Responses In Vitro.- Recombinant Poxviruses: Versatile Tools for Immunological Assays.- Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy.- Monitoring MHC Ubiquitination by MARCH Ubiquitin Ligases.- Monitoring MHC-II Endocytosis and Recycling using Cell-Surface Protein Biotinylation-Based Assays.- Monitoring Protein Endocytosis and Recycling using FACS-Based Assays.- In Vitro Digestion with Proteases Producing MHC Class II Ligands.- Assembly, Intracellular Transport and Release of MHC Class II Peptide Receptors.- Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading.- Studying MHC Class II Peptide Loading andEditing In Vitro.- Monitoring Antigen Processing for MHC Presentation via Macroautophagy.- Identification of Human Antigen-Specific CD4+ T-Cells with Peptide-MHC Multimer Technologies.- Production of Antigen Specific Human CD4+ T Cell Lines and Clones.- Monitoring Dendritic Cell Activation and Maturation.- Studying MHC Class II Presentation of Immobilized Antigen by B Lymphocytes.- Analyzing Antigen Recognition by Natural Killer T Cells.