Del i serien Methods in Molecular Biology
Animal Cell Electroporation and Electrofusion Protocols
Häftad, Engelska, 2013
709 kr
Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt för medlemmar vid köp för minst 249 kr.
The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.
Produktinformation
- Utgivningsdatum2013-08-11
- Mått152 x 229 x 22 mm
- Vikt566 g
- FormatHäftad
- SpråkEngelska
- SerieMethods in Molecular Biology
- Antal sidor370
- Upplaga1995
- FörlagHumana Press Inc.
- ISBN9781489940612
Tillhör följande kategorier
Hoppa över listan
Du kanske också är intresserad av
Omvårdnadens grunder - paket
Sofia Almerud, Annica Backman, Anna-Karin Edberg, Anna Ehrenberg, My Engström, Katarina Göransson, Lena Marmstål Hammar, Stefan Nilsson, Lars Wallin, Helle Wijk, Joakim Öhlén, Lilas Ali, Anette Alvariza, Ann-Christine Andersson, Pia Andersson, Anna Anåker, Charlotta van Eggermont Arwidson, Margareta Asp, Åsa Audulv, Malin Axelsson, Mariette Bengtsson, Eva Benzein, Lena Berg, Monica Bergqvist, Eva-Karin Bergström, Ida Björkman, Catrin Björvell, Angela Bång, Carina Bååth, Josefin Bäckström, Karin Bölenius, David Edvardsson, Rakel Eklund, Inger Ekman, Mirjam Ekstedt, Ann Catrine Eldh, Karin Enskär, Sofia Erestam, Anneli Eriksson, Henrik Eriksson, Marie Ernsth Bravell, Jan Florin, Anna Forsberg, Henrietta Forsman, Anette Forss, Mio Fredriksson, Febe Friberg, Maria Grandahl, Tanja Gustafsson, Agneta Gånemo, Elizabeth Hanson, Malin Hansson, Amanda Hellström, Ingela Henoch, Inger Jansson, Maria Jirwe, Lena Johansson, Pauline Johansson, Unn-Britt Johansson, Birgitta Kerstis, Ann-Sofie Källberg, Ulrika Källman, Anita Kärner Köhler, Cecilia Larsdotter, Margret Lepp, Maria Lindström Nilsson, Mikael Lundmark, Anneli Löf, Lennart Magnusson, Catharina Melander, Jenny Milton, Beata Molin, Annika Nordin, Maria Norinder, Sepideh Olausson, Carina Persson, Kerstin Prignitz Sluys, Josefin Rahmqvist, Birgit Holritz Rasmussen, Anneli Ringblom, Karin C. Ringsberg, Ann Rudman, Ann-Marie Rydholm Hedman, Silje Rysst, Lars Sandman, Regina Santamäki Fischer, Britt-Inger Saveman, Johanna Sjömar, Karin Stenzelius, Anna Strömberg, Annelie J. Sundler, Märtha Sund Levander, Kristina Svantesson, Anna Swall, Susanne Syrén, Pia Tingström, Johanna Ulfvarson, Sara Wallström, Albert Westergren, Lena Wiklund Gustin, Ulrika Winblad
Häftad, 2025
1 569 kr