Animal Cell Culture

Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.

• Contributors. Preface.Abbreviations.Protocols.1 The Cell Culture Laboratory (Sue Clarke and Janette Dillon).2 Sterilization (Peter L. Roberts).3 Microscopy of Living Cells (Colin Gray and Daniel Zicha).4 Basic Techniques and Media, the Maintenance of Cell Lines, and Safety (John M. Davis).5 Development and Optimization of Serum- and Protein-free Culture Media (Stephen F. Gorfien and David W. Jayme).6 Cryopreservation and Banking of Cell Lines (Glyn N. Stacey, Ross Hawkins and Roland A. Fleck).7 Primary Culture of Specific Cell Types and the Establishment of Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, David Tai Wei Leong, Chris Morris and Jan-Thorsten Schantz).8 Cloning (John Clarke, Alison Porter and John M. Davis).9 The Quality Control of Animal Cell Lines and the Prevention, Detection and Cure of Contamination (Peter Thraves and Cathy Rowe).10 Systems for Cell Culture Scale-up (Jennifer Halsall and John M. Davis).11 Good Laboratory Practice in the Cell Culture Laboratory (Barbara Orton).Index.Colour plates.

"It will be a great resource for students and established researchers who want to learn more about modern cell culture techniques." (Doody's, 13 January 2012)