Angiogenesis

Methods and Protocols

Häftad, Engelska, 2023

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This detailed book builds on the foundation of lymphatic protocols with tools to aid in the interpretation of large datasets created by angiogenesis research.

Produktinformation

Utgivningsdatum2023-02-02
Mått178 x 254 x 25 mm
Vikt837 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor429
FörlagSpringer-Verlag New York Inc.
ISBN9781071620618

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik

The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo.- A Workflow to Track and Analyze Endothelial Migration during Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy.- Analyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling.- Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of 4th Pharyngeal Arch Artery Formation.- Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina.- Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin.- Analyzing Lymphatic Vessel Patterning in Adult Tissue.- Transmission Electron Microscopy of Endothelium.- Measurement of Revascularisation in the Hind Limb after Experimental Ischemia in Mice.- Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts.- Non-Invasive Measurement of Retinal Microvascular Permeability during Loss of Endothelial Quiescence.- An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery.- Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart.- Quantifying Lymphatic Vessel Density in Human Tissue Samples.- Quantifying Vascular Remodeling in the Mouse Spinal Cord.- Quantitative Methods to Assess Adipose Vasculature.- Quantification of Angiogenesis in Laser Choroidal Neovascularization.- A Rapid Adaptable Method for Isolation of Endothelial Cells from Human Adipose Tissue.- Simple Gene Knockdown in Endothelial Cells Using Short Interfering RNA Oligonucleotides.- Transcription Factor Chromatin Immunoprecipitation in Endothelial Cells.- In Vitro Co-Culture of Fibroblast and Endothelial Cells to Assess Angiogenesis.- Quantification of Leukocyte-Endothelial Cell Interaction during In Vitro Models of Thrombosis at Arterial and Venous Shear Rates.- Isolation of Cardiac Endothelial Cells forTranscriptomic Analysis of the Zebrafish and Mouse Heart.- The Evaluation of Neovessel Angiogenesis Behavior at Tissue Interfaces.- Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.- Live Cell Imaging and Analysis of Cancer-Cell Transmigration through Endothelial Monolayers.- Differentiation and Characterization of Human Pluripotent Stem Cell-Derived Cardiac Endothelial Cells for In Vitro Applications.- Finding and Verifying Enhancers for Endothelial-Expressed Genes.- Endothelial Cell RNA-Seq Data: Differential Expression and Functional Enrichment Analyses to Study Phenotypic Switching.