Ancient DNA

Methods and Protocols

Häftad, Engelska, 2016

1 569 kr

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Research into ancient DNA began more than 25 years ago with the publication of short mitochondrial DNA sequence fragments from the quagga, an extinct relative of the zebra.

Produktinformation

Utgivningsdatum2016-08-23
Mått178 x 254 x 15 mm
Vikt496 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor247
FörlagHumana Press Inc.
ISBN9781493958740

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Setting Up An Ancient DNA Laboratory.-A Phenol-Chloroform Protocol for Extracting DNA from Ancient Samples.-DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica Particles.-Case Study: Recovery of ancient nuclear DNA from Toe Pads of the Extinct Passenger Pigeon.-Extraction of DNA from Paleofeces.-DNA Extraction from Keratin and Chitin.-Case Study: Ancient sloth DNA Recovered from Hairs Preserved in Paleofeces.-Ancient DNA Extraction from Soils and Sediments.-DNA Extraction from Fossil Eggshell.-Ancient DNA Extraction from Plants.-DNA Extraction from Formalin-fixed Material.-Case Study: Ancient DNA recovered from Pleistocene-age Remains of a Florida Armadillo.-Non-destructive DNA Extraction from Museum Specimens.-Case Study: Using a Non-destructive DNA Extraction Method to Generate mtDNA Sequences from Historical Chimpanzee Specimens .-PCR Amplification, Cloning and Sequencing of Ancient DNA.-Quantitative Real-time PCR in aDNA Research.-Multiplex PCR Amplification of Ancient DNA.-Preparation of Next-generation Sequencing Libraries from Damaged DNA.-Generating Barcoded Libraries for Multiplex High-throughput Sequencing.-Case Study: Targeted high-throughput Sequencing of Mitochondrial Genomes from Extinct Cave Bears via Direct Multiplex PCR Sequencing (DMPS).-Target Enrichment via DNA Hybridization Capture.-Case Study: Enrichment of Ancient Mitochondrial DNA by Hybridization Capture.-Analysis of High-throughput Ancient DNA Sequencing Data.-Phylogenetic Analysis of Ancient DNA using BEAST.