Analytical Ultracentrifugation

Dr. Cole received a Sc.B. in Biochemistry from Brown University. He obtained his Ph.D. in Biophysical Chemistry at UC Berkeley where he investigated the mechanism of photosynthetic oxygen evolution. He carried out postdoctoral research at Stanford University on the structure and function of metalloenzymes that interact with oxygen. In 1991, he took a position at Merck Research Laboratories in antiviral drug discovery. Dr. Cole applied analytical ultracentrifugation and related biophysical techniques to probe macromolecular interactions that regulate replication of HIV, Influenza and Hepatitis C virus. In 2001, he to return to academia and moved to the Department of Molecular and Cell Biology at the University of Connecticut, where he is professor of structural biology, biophysics and biochemistry. His research group uses biophysical approaches to characterize macromolecular interactions that regulate important biological processes. A major focus is the innate immunity pathway for defense against viral infection. In an NIH-supported research program, his group has defined the mechanism for activation of protein kinase R by RNA. He directs the National Analytical Ultracentrifugation Facility and holds a joint appointment in the department of Chemistry at the University of Connecticut. He is a member of the editorial board of Biophysical Journal. He is the author of 84 publications and reviews.

• 1. Next Generation AUC adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical UltracentrifugeJoseph Pearson, Frank Krause, Dirk Haffke, Borries Demeler, Kristian Schilling and Helmut Cölfen2. Next Generation AUC: Analysis of Multi-Wavelength Analytical Ultracentrifugation DataGary E. Gorbet, Joseph Z. Pearson, Aysha K. Demeler, Helmut Cölfen and Borries Demeler3. Sedimentation Velocity: A Classical PerspectiveJohn J. Correia and Walter F. Stafford4. Hydrodynamic Modeling and its Application in AUCMattia Rocco and Olwyn Byron5. Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation DataChad A. Brautigam6. Sedimentation Equilibrium Analysis of Clpb Self-Association in Diluted and Crowded Solutions. Carlos Alfonso, Urko del Castillo, Ianire Martín, Arturo Muga and Germán Rivas7. Analysis of Linked EquilibriaJiaBei Lin and Aaron L. Lucius8. Elucidating Complicated Assembling Systems in Biology Using Size-And-Shape Analysis of Sedimentation Velocity DataCatherine T. Chaton and Andrew B. Herr9. Quaternary Structure Analyses of an Essential Oligomeric EnzymeTatiana P. Soares da Costa, Janni B. Christensen, Sebastien Desbois, Shane E. Gordon, Ruchi Gupta, Campbell J. Hogan, Tao G. Nelson, Matthew T. Downton, Chamodi K. Gardhi, Belinda M. Abbott, John Wagner, Santosh Panjikar and Matthew A. Perugini10. Characterization of Intrinsically Disordered Proteins by Analytical UltracentrifugationDavid J. Scott and Donald J. Winzor11. Sedimentation Velocity Analysis of the Size Distribution of Amyloid Oligomers and FibrilsYee-Foong Mok, Geoffrey J. Howlett and Michael D.W. Griffin12. AUC and Small Angle Scattering for Membrane ProteinsAline Le Roy, Kai Wang, Béatrice Schaack, Peter Schuck, Cécile Breyton and Christine Ebel13. Hydrodynamic Models of G-quadruplex StructuresJonathan B. Chaires, William L. Dean, Huy Le and John O. Trent14. Analytical Ultracentrifugation as a Tool to Study Non-specific Protein-DNA InteractionsTeng-Chieh Yang, Carlos Enrique Catalano and Nasib Karl Maluf15. Characterization of Homogeneous, Cooperative Protein-DNA Clusters by Sedimentation Equilibrium Analytical Ultracentrifugation and Atomic Force MicroscopyIngrid Tessmer and Michael G. Fried16. Sedimentation Velocity Analysis of Large Oligomeric Chromatin Complexes Using Interference Detection.Hansen and Jeffrey17. Methods in Enzymology – Analytical UltracentrifugationDavid L Bain, Rolando W De Angelis, Keith D Connaghan, Qin Yang, Gregory D Degala and James R Lambert 18. Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates and LigninsStephen E. Harding, Gary G. Adams, Fahad Almutairi, Qushmua Alzahrani, Tayyibe Erten, M. Samil Kök and Richard B. Gillis19. Analytical Ultracentrifugation and its Role in Development and Research of Therapeutical Proteins.Jun Liu, Sandeep Yadav, James Andya, Barthélemy Demeule and Steven J. Shire20. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUCKelly K. Arthur, Brent S. Kendrick and John P. Gabrielson21. Protein Assembly in Serum and the Differences from Assembly in Buffer.John J. Hill and Thomas M. Laue

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