This two-volume set LNAI 16370-16371 constitutes the refereed proceedings of the 38th Australasian Joint Conference on Artificial Intelligence, AI 2025, held in Canberra, ACT, Australia, during December 1–5, 2025.The 73 full papers presented together with 1 short papers were carefully reviewed and selected from 152 submissions. They were organized in following topical section:Part I :  Language and Foundation Models and Knowledge Representation and Data Mining,Part II : Learning Algorithms, Computer Vision, AI for Healthcare and  Reinforcement Learning and Robotics.