Dr. Vijai Singh is an Associate Professor at the Department of Biosciences, Indrashil University, Mehsana, Gujarat, India. He previously served as a Postdoctoral Fellow at the Institute of Systems and Synthetic Biology, France and at the School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, South Korea. Dr. Singh has also served as an Assistant Professor at Department of Biotechnology, Invertis University, India and the Department of Biological Sciences and Biotechnology, Institute of Advanced Research, India. He received his Ph.D. in Biotechnology from Dr. A.P.J. Abdul Kalam Technical University/National Bureau of Fish Genetic Resources, Lucknow, India.Dr. Singh has designed and characterized a number of synthetic oscillators, gene regulatory networks, and biosynthetic pathways in Escherichia coli. Currently, his laboratory is focusing on the construction of a novel biosynthetic pathway for the production of pigments and chemicals. Additionally, his laboratory works on development of CRISPR-based platform for the diagnosis and eradication of multidrug resistant pathogens. Dr. Singh has published 72 articles, 23 book chapters, and two books. He currently serves as a member of the editorial board and reviewer for a number of peer-reviewed journals.

• Chapter 1. Introduction to Synthetic Biology.- Chapter 2. Current progress in synthetic genetic networks.- Chapter 3. Current Progress and Limitations in the Design, Construction and Characterization of Synthetic Parts.- Chapter 4. Recent progress in DNA parts standardization and characterization.- Chapter 5. Current status and challenges of DNA sequencing.- Chapter 6. Biomimetic approaches in synthetic biology.- Chapter 7. Design principles of synthetic biological oscillators.- Chapter 8. SOFTWARE-AIDED DESIGN OF IDEALISED PROGRAMMABLE NUCLEIC ACID CIRCUITS.- Chapter 9. Digital Circuit Design for Biological and Silicon Computers.- Chapter 10. Engineering of riboregulators for gene regulation as a tool for synthetic biology.- Chapter 11. Recent advances, challenges and opportunities in riboswitches.- Chapter 12. Recent Advances in Gene and Genome Assembly: Challenges and Implications.- Chapter 13. Recent advances, challenges and opportunities in synthetic genomes.- Chapter 14. Expansion of the genetic code.- Chapter 15. Expanding the potential of CRISPR-Cas9 technology for crops improvement.- Chapter 16. Synthetic biology at the hand of cell-free systems.- Chapter 17. Synthetic Biology for the Rapid, Precise and Compliant Detection of Microbes.- Chapter 18. Application and challenges of synthetic biology.- Chapter 19. Development and Application of Microfluidics in Synthetic Biology.- Chapter 20. The Ethics of Synthetic Biology Research and Development: A Principlist Approach.