Advanced Methods in Molecular Biology and Biotechnology

Khalid Z. Masoodi is an Associate Professor and Senior Scientist in the Division of Plant Biotechnology at Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India. He is the recipient of a number of awards and holds numerous patents. He has two decades worth of teaching and research experience in the areas of plant biotechnology, molecular biology, cancer research, and human disease research, and has published numerous articles and books. He leads a transcriptomics laboratory and works extensively with fluorescence imaging, real-time PCR, cell culture, and Western blotting platforms. His scientific interests include transcription biology in plant stress responses and cancer, as well as the discovery of new drugs from medicinal plants.Sameena Maqbool Lone is a biotechnology researcher with a strong focus on vegetable science, molecular biotechnology, and sustainable bioproduction. She is currently a postdoctoral researcher in the Division of Vegetable Science, Sher-e- Kashmir University of Agricultural Sciences and Technology of Kashmir, India. Her current work explores the modulation of inflammatory and oncogenic signalling pathways, positioning plant-derived compounds within the broader framework of translational cancer therapeutics. Her research interests include phytochemical science, molecular diversity analysis, and the application of plant resources for health-related innovation. She holds a number of patents and has co-authored several research papers and books. Rovidha Saba Rasool did her Bachelors in Agriculture from SKUAST-K, Wadura followed by Masters in Plant Pathology from SKUAST-Kashmir, Shalimar. Currently, she is pursuing her Ph.D from Division of Plant Pathology, SKUAST-Kashmir. She has done commendable work in finding new genes involved in the pathogenesis of shot hole in stone fruits through insertional mutagenesis in Thyrostroma carpophilum. She has been a good student holding an excellent academic and extracurricular record and was awarded Ph.D. merit scholarship by SKUAST-K, in 2018-19. Apart from this, Rovidha has taken part in various national-level sports competitions and has won many national and state-level awards in baseball and handball.

• 1. Introduction to molecular biology techniques2. Agarose gel electrophoresis3. SDS-polyacrylamide gel electrophoresis4. Immunoelectrophoresis5. DNA plasmid isolation using Alkaline Lysis Method from E. coli DH5α cells6. Restriction digestion of Plasmid DNA7. Genomic DNA extraction from plant leaves using CTAB method8. Genomic DNA extraction from silkworm using modified phenol: chloroform method (Suzuki et al., 1972)9. Genomic DNA extraction from Fungal Mycelium using modified Cenis, 1992 method10. Bacterial transformation protocol using CaCl211. Transformation of E. coli by electroporation12. Transfection of cells using lipofectamine13. Total RNA isolation14. RNA isolation from plant tissues with high phenolic compounds and polysaccharides15. Total protein extraction with TCA-acetone and 2D gel electrophoresis16. Dansyl-Edman method of peptide sequencing17. ISSR analysis18. Gene cloning/recombinant DNA technology19. Polymerase Chain Reaction (PCR)20. Blotting/ hybridization and its types21. Dot blot analysis22. ELISA (enzyme-linked immunosorbent assay)23. Study of principle of centrifugation24. Study of principle of chromatography25. Thin layer chromatography (TLC)26. Gel-filtration or size-exclusion chromatography27. Ion-exchange chromatography28. To perform affinity chromatography29. To perform paper chromatography30. Growth of bacterial cultures and preparation of growth curve31. To study standard operating procedures (SOPs) for various equipment