Adipogenic differentiation from human embryonic stem cells through formation of embryoid bodies.- Quantification of adipogenesis using flow cytometry.- Isolation of Wharton’s Jelly derived Mesenchymal Stromal/Stem Cells.- Adipogenic differentiation assay for the study of insulin signalling.- Quantification of lipid droplets in adipocytes.- RT-qPCR quantification of adipogenic marker genes (Pparg, C/ebpa, Fabp4 and Cd36).- Adipogenic differentiation of human adipose-derived mesenchymal stromal/stem cells (ASCs).- Western blot detection of adipogenic protein.- Measurement of intracellular Reactive Oxygen Species during adipogenesis.- Oxygen consumption and extra cellular acidification rate in adipogenic differentiated human mesenchymal stromal/stem cells.- In vitro expansion of human adipose-derived stem cells (ASCs).- siRNA silencing of adipogenic gene in ASCs.- Freezing and thawing of Adipose-derived stromal/stem cells (ASCs).- Isolation and Characterization of Mouse Adipose-Derived Stromal/Stem Cells.- Isolation of adipose-derived mesenchymal stromal/stem cells.- Isolation and purification of adipocyte-derived extracellular vesicles (AdEVs) using size-exclusion chromatography.- Differentiation of Adipose derived stromal/stem cells into Osteocytes.- Differentiation of mesenchymal stromal/stem cells from induced pluripotent stem cells.- Isolation and Characterization of Equine Mezenchymal Stromal/Stem Cell.
