Activity-Based Protein Profiling

Benjamin F. Cravatt, Ph.D.Department of ChemistryThe Scripps Research InstituteLa Jolla, CA, USAcravatt@scripps.edu Ku-Lung Hsu Ph.D.University of Virginia School of MedicineDepartment of ChemistryCharlottesville, VA, USAkh4ch@virginia.edu Eranthie Weerapana Ph.D.Boston CollegeMerkert ChemistryChestnut Hill, MA, USAeranthie@bc.edu

• Activity-based protein profiling – enabling multimodal functional studies of microbial communities.- Activity-Based Protein Profiling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles.- Opportunities and Challenges in Activity-Based Protein Profiling of Mycobacteria.- Activity-Based Protein Profiling at the Host–Pathogen Interface.- Chemical Proteomic Profiling of Protein Fatty-Acylation in Microbial Pathogens.- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling.- ABPP and Host–Virus Interactions.- Activity-Based Protein Profiling for the Study of Parasite Biology.- Deciphering T Cell Immunometabolism with Activity-Based Protein Profiling.- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics.- Development of Activity-Based Proteomic Probes for Protein Citrullination.- Recent Advances in Activity-Based Protein Profiling of Proteases.- Opportunities for Lipid-Based Probes in the Field of Immunology.- Activity-Based Protein Profiling of Non-ribosomal Peptide Synthetases.- Target Identification of Bioactive Covalently Acting Natural Products.- Applications of Reactive Cysteine Profiling.