A Laboratory Guide to the Mammalian Embryo

• Preface ; Acknowledgements ; List of Contributors ; Abbreviations ; 1. Follicle development in vitro ; 2. Preparation of gametes, in vitro maturation, in vitro fertilization and embryo recovery ; 3. Culture of the mammalian preimplantation embryo ; 4. Assessment of embryo development and viability ; 5. Micromanipulation: ICSI ; 6. Micromanipulation: Biopsy ; 7. Analysis of intracellular ions: pH and calcium ; 8. Nutrient uptake and metabolite production and enzyme activity and regulation ; 9. Metabolic Pathway ; 10. Confocal imaging of structural molecules in mammalian gametes ; 11. Fluorescent in situ hybridization ; 12. Assessment of ploidy, telomere length and telomerase activity in oocytes and embryos ; 13. Microscale RNA isolation from mammalian embryos ; 14. Relative mRNA transcript abundance in early embryos by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) ; 15. Characterization of novel genes during early development by application of differential display RT-PCR ; 16. Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells ; 17. Antisense disruption of gene expression in the preimplantation embryo ; 18. Gene subtraction and analysis ; 19. Analysis of apoptosis in the preimplantation embryo ; 20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo ; 21. Human Embryonic Stem ; 22. Spermatogonial Transplantion ; 23. Somatic Cell Nuclear Transfer ; 24. Cryobiology; slow freezing vitrification of embryos

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