3D Sponge-Matrix Histoculture

Robert M. Hoffman, Ph.D. Professor of Surgery, University of California, San Diego President, AntiCancer, Inc. 7917 Ostrow Street San Diego, CA 92111, USA

• In Memorium: Joseph Leighton, 1921-1999 – Father of 3-Dimensional Tissue Culture.- 3D Sponge-Matrix Histoculture: An Overview.- In Vivo-Like Growth Patterns of Multiple Types of Tumors in Gelfoam® Histoculture.- Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology.- Development of the Histoculture Drug Response Assay (HDRA).- Diagnosis and Pathological Analysis of Patient Cancers by Detection of Proliferating Cells in Gelfoam® Histoculture.- Clinical Correlation of the Histoculture Drug Response Assay in Gastrointestinal Cancer.- Prospective Clinical Correlation of the Histoculture Drug Response Assay for Ovarian Cancer.- Clinical Correlation of the Histoculture Drug Response Assay for Head and Neck Cancer.- Clinical Usefuleness of the Histoculture Drug Response Assay for Breast Cancer.- Clinical Usefuleness of the Histoculture Drug Response Assay for Prostate Cancer and Benign Prostate Hypertrophy (BPH).- In Vivo-Like Cell Cycle Phase Distribution of Cancer Cells in Gelfoam® Histoculture Observed in Real-Time by FUCCI Imaging.- Methionine Dependency Determination of Human Patient Tumors in Gelfoam® Histoculture.- Hair-Shaft Growth in Gelfoam® Histoculture on Skin and Isolated Hair Follicles.- Histoculture of Hair-Follicle-Associated Pluripotent (HAP) Stem Cells in Gelfoam® Histoculture for Use in Spinal Cord Repair.- Nerve Growth and Interaction in Gelfoam® Histoculture: A Nervous System Organoid.- Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam®.- Imaging DNA Repair after UV Irradiation Damage of Cancer Cells in Gelfoam® Histoculture.- Comparison of “Dimensionality” of Cancer Cell Culture in Gelfoam® Histoculture and Matrigel.- Imaging the Governing Step of Metastasis in Gelfoam® Histoculture.