3D Bioprinting and Bioinks for Regenerative Engineering

Anthony Atala, MD, is the G. Link Professor and Director of the Wake Forest Institute for Regenerative Medicine. Seventeen of his lab’s technologies have been translated to patients, including the first tissue-engineered organ for permanent implantation in a human. His research led to the discovery of a multipotent source of stem cells from amniotic fluid and placenta, advances in 3D printing of tissues and organs, and body-on-a-chip technologies. His work was twice listed among Time Magazine’s top 10 medical breakthroughs of the year, and he is a recipient of the Smithsonian Ingenuity Award. He has published over 900 journal articles, is the editor of 25 books, and holds over 250 national and international patents. Dr. Atala is a member of the National Academy of Medicine, the National Academy of Inventors, and the American Institute for Medical and Biological Engineering.Dr. Lim’s research interests encompass tissue engineering, biofabrication, regenerative engineering, and controlled drug delivery systems. Her work has been widely recognized, with over 150 peer-reviewed publications and numerous patents, some of which have been licensed to companies. Dr. Lim has been honored with prestigious awards such as the TERMIS International Fellow Award and KTERMS Award, highlighting her contributions to the field. Dr. Lim has delivered over 160 invited presentations worldwide and has played key roles in various academic and professional organizations, including serving as President of the Korea Tissue Engineering and Regenerative Medicine Society and as a TERMIS Fellow. Her editorial roles span several leading journals, including Tissue Engineering and Regenerative Medicine, where she has served as an Editorial Board Member and Associate Editor. Dr. Lim has held academic appointments at Wake Forest University School of Medicine, where she is currently a Professor in the Department of Regenerative Medicine.

• Part I: Introduction1. History and Evolution of 3D Printing and Bioinks in Regenerative EngineeringPart II: 3D Printing Technologies2. Extrusion-Based Printing3. Jetting-Based Printing4. Light-Based Printing5. Hybrid Printing6. Automation and Robotics in Bioprinting7. Multiphoton Lithography and Emerging PrecisionPart III: Fundamentals of Bioinks for 3D Printing8. Introduction to Bioinks9. Nature-Derived Hydrogel Bioinks10. Synthetic Polymer Bioinks11. Composite Bioinks12. Bioink Formulations and Design13. Self-Assembling Bioinks14. Cellular Aggregates ad Spheroids as Building Blocks15. Green Processing and Lifecycle Considerations for Bioinks16. Preservation and Sterilization of Bioinks17. Manufacturing and Translational Perspectives of BioinksPart IV: Printing Tissue and Organ Constructs18. Skin19. Cartilage20. Muscle21. Bone22. Kidney23. Pancreas24. Liver25. Lung26. Trachea27. Heart28. Brain29. Gastrointestinal Structures30. Craniofacial Structures31. Oral Cavity32. Reproductive Structures33. Urologic Structures34. Vascular Structures35. Immune Systems36. NervePart V: Regulatory Considerations and Future Perspectives37. Regulatory Pathways for 3D Bioprinted Products38. Ethical and Societal Dimensions of 3D Printing39. Innovations in Body-on-a-Chip Systems40. Bioprinting in Space: Opportunities and Challenges