Del i serien Methods in Molecular Biology

Molecular Methods in Developmental Biology

Xenopus and Zebrafish

Inbunden, Engelska, 1999

1 379 kr

Beställningsvara. Skickas inom 10-15 vardagar. Fri frakt för medlemmar vid köp för minst 249 kr.

This book is primarily aimed at newcomers to the field of developmental biology, but is also useful to experienced workers. In this volume, the South African clawed frog Xenopus Iaevis and the zebrafish Brachydanio rerio serve as the model organisms for the developmental studies. Both species are well suited to investigations of early vertebrate development because of their embryos develop rapidly and externally, but each also has its set of disadvantages. This methods manual includes experimental approaches which can be applied to both species, since many laboratories also use these two models for similar studies. For example, the approaches for the analysis of steady-state mRNA levels by RT-PCR or PNase protection are identical for Xenopus and zebrafish, but for whole-mount in situ hybridization, two tissue explant and transplant techniques, quantitative and spatial analysis of mRNA and immunohistochemistry. The final chapters focus on more specialized methods for expression and promoter analysis, such as the analysis of transcription control in early development.The methods and techniques presented in this volume will provide a sound basis for the majority of studies in this field.

Produktinformation

Utgivningsdatum1999-08-03
Mått155 x 235 x 20 mm
Vikt560 g
FormatInbunden
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor217
Upplaga1999
FörlagHumana Press Inc.
ISBN9780896037908

Tillhör följande kategorier

The Animal Cap Assay.- Cell and Tissue Transplantation in Zebrafish Embryos.- Ribonuclease Protection Analysis of Gene Expression in Xenopus.- Quantitative Analysis of mRNA Levels in Xenopus Embryos by Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR).- Wholemount In Situ Hybridization of Xenopus and Zebrafish Embryos.- In Situ Hybridization to Sections of Xenopus Embryos.- Zebrafish Immunohistochemistry.- Immunohistochemistry of Xenopus Embryos.- Preparation and Testing of Synthetic mRNA for Microinjection.- Microinjection into Xenopus Oocytes and Embryos.- Microinjection into Zebrafish Embryos.- Expression from DNA Injected into Xenopus Embryos.- Promoter Analysis in Zebrafish Embryos.- Transient Transgenesis in Xenopus laevis Facilitated by AAV-ITRs.- Band-Shift Analysis Using Crude Oocyte and Embryo Extracts from Xenopus laevis.- DNA-Footprinting using Crude Embryonic Extracts from Xenopus laevis.- Mapping Protein-DNA Interactions Using In Vivo Footprinting.

"Readers will find the systematic description of methods, illustration of structures, tables and figures, along with comments on optimizing the methods for use in other systems, very useful. ...this excellent book will facilitate studies on the lipidation of proteins. It should be useful to both beginning and experienced investigators in the field." - Analytical Biochemistry "...this compact book comprises concise and very well structured chapters on both basic and advanced molecular and embryological technique used in studies of early stages of vertebrate development on two model species, the South Africa clawed frog Xenopus loevis and the zebrafish Brachydanio rerio...All chapters have been written by authors with first-hand expertise in individual fields, mostly those in whose laboratories the methods in question have been developed, or at least optimized. Close attention is paid to meticulous description of possible pitfalls and drawbacks, and essential steps are stressed...the book contains a wealth of information, cues on methodology and their critical assessment. It is an almost indispensable guide for anybody working in the field of vertebrate development biology."-Folia Microbiologica "...this book is a solid introduction to some of the common techniques in zebrafish and Xenopus and I think would be of good use to its intended audience of new workers in the field." - Newsletter of the British Society of Developmental Biology