Inflammasome

Methods and Protocols

Inbunden, Engelska, 2022

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This volume provides inflammasome instructions and tips to study different aspects of inflammasome biology.

Assessment of ASC Oligomerization by Flow Cytometry.- In-vitro Assays to Study Inflammasome Activation in Primary Macrophages.- Measuring Non-Canonical Inflammasome Activation In Neutrophils.- Gasdermin D Cleavage Assay Following Inflammasome Activation.- Activation of the Non-Canonical Inflammasome In Mouse And Human Cells.- Measurement of Inflammasome-Induced Mitochondrial Dysfunction By Flow Cytometry.- Detection of ASC Oligomerization by Western Blotting.- Reconstitution System of NLRP3 Inflammasome in HEK293T Cells.- Intracellular Potassium Ions Measurements by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES).- NLRP3 Phospho-Residue Mapping by Phospho Dot-Blots.- Analysis of Activity and Expression of the NLRP3, AIM2 and NLRC4 Inflammasome in Whole Blood.- Measurement of Cytosolic Mitochondrial DNA after NLRP3 Inflammasome Activation.- Inflammasome-derived Exosomes Isolation and Imaging by Transmission Electron Microscopy.- In Vitro and In Vivo Model for Sepsis through Non-Canonical NLRP3 inflammasome Activation.- Inflammasome Activation in Gingival Epithelial Cells.- Imaging of Inflammasome Speck Formation in Living Cells.