Benson's Microbiological Applications Complete Version

Professor and Chair, Consultant Environmental Microbiology Heidi Smith leads the microbiology discipline at Front Range Community College in Fort Collins, CO, teaching a variety of biology courses each semester including microbiology, anatomy/ physiology, and biotechnology Collaboration with other faculty across the nation, the development and implementation of new digital learning tools, and her focus on student learning outcomes have revolutionized her face-to-face and online teaching approaches and student performance in her classes. Outside of the classroom, Heidi oversees a federal grant program designed to train and support underrepresented students for undergraduate research and transfer into four-year STEM degree programs. She is also an active member of the American Society for Microbiology and participated as a task force member for the development of their Curriculum Guidelines for Undergraduate Microbiology Education. Off campus, Heidi enjoys the beautiful Colorado outdoors with her husband and three young children.

• Part 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Fluorescence Microscopy5 Microscopic MeasurementsPart 2 Survey of Microorganisms6 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria7 Ubiquity of Bacteria8 The Fungi: Molds and YeastsPart 3 Manipulation of Microorganisms9 Aseptic Technique10 Pure Culture TechniquesPart 4 Staining and Observations of Microorganisms11 Smear Preparation12 Simple Staining13 Negative Staining14 Capsular Staining15 Gram Staining16 Spore Staining: Two Methods17 Acid-Fast Staining: Kinyoun Method18 Motility DeterminationPart 5 Culture Methods19 Culture Media Preparation20 Preparation of Stock Cultures21 Enumeration of Bacteria: The Standard Plate Count22 Slide Culture: FungiPart 6 Bacterial Viruses23 Determination of a Bacteriophage Titer24 A One-Step Bacteriophage Growth Curve25 Isolation of Phage from Flies26 Phage Typing Part 7 Environmental Influences and Control of Microbial Growth27 Effects of Oxygen on Growth28 Temperature: Effects on Growth29 pH and Microbial Growth30 Water Activity and Osmotic Pressure31 Ultraviolet Light: Lethal Effects32 The Effects of Lysozyme on Bacterial Cells33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method35 Evaluation of Antiseptics: The Filter Paper Disk Method36 Effectiveness of Hand ScrubbingPart 8 Identification of Unknown Bacteria37 Morphological Study of An Unknown Bacterium38 Cultural Characteristics39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions41 Physiological Characteristics: Multiple Test Media42 Use of Bergey’s ManualPart 9 Miniaturized Multitest Systems43 Enterobacteriaceae Identification: The API 20E System44 Enterobacteriaceae Identification: The Enterotube II System45 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System46 Staphylococcus Identification: The API Staph SystemPart 10 Diversity and Environmental Microbiology47 Isolation of an Antibiotic Producer: The Streptomyces48 Nitrogen Cycle: Ammonification49 Symbiotic Nitrogen Fixation: Rhizobium50 Free-Living Nitrogen Fixation: Azotobacter51 Denitrification: Paracoccus denitrificans52 The Winogradsky Column53 Purple Nonsulfur Photosynthetic Bacteria54 Sulfate Reducing Bacteria: Desulfovibrio55 Bacterial Commensalism56 Bacterial Synergism57 Microbial Antagonism Part 11 Applied Microbiology58 Bacterial Food Counts59 Bacteriological Examination of Water: Most Probable Number Determination60 Bacteriological Examination of Water: The Membrane Filter Method61 Reductase Test62 Temperature: Lethal Effects63 Microbial Spoilage of Canned Food64 Microbiology of Alcohol FermentationPart 12 Bacterial Genetics and Biotechnology65 Mutant Isolation by Replica Plating66 Bacterial Transformation67 Polymerase Chain Reaction for Amplifying DNA68 Plasmid IsolationPart 13 Medical Microbiology69 The Staphylococci: Isolation and Identification70 The Streptococci and Enterococci: Isolation and Identification71 Gram-Negative Intestinal Pathogens72 A Synthetic EpidemicPart 14 Immunology and Serology73 Slide Agglutination Test: Serological Typing74 Slide Agglutination Test for S. aureus75 Slide Agglutination Test for Streptococcus76 The Heterophile Antibody Test77 Blood Grouping Appendices