Benson's Microbiological Applications, Laboratory Manual in General Microbiology, Short Version

Professor and Chair, Consultant Environmental Microbiology Heidi Smith leads the microbiology discipline at Front Range Community College in Fort Collins, CO, teaching a variety of biology courses each semester including microbiology, anatomy/ physiology, and biotechnology Collaboration with other faculty across the nation, the development and implementation of new digital learning tools, and her focus on student learning outcomes have revolutionized her face-to-face and online teaching approaches and student performance in her classes. Outside of the classroom, Heidi oversees a federal grant program designed to train and support underrepresented students for undergraduate research and transfer into four-year STEM degree programs. She is also an active member of the American Society for Microbiology and participated as a task force member for the development of their Curriculum Guidelines for Undergraduate Microbiology Education. Off campus, Heidi enjoys the beautiful Colorado outdoors with her husband and three young children.

• Part 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Microscopic MeasurementsPart 2 Survey of Microorganisms5 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria6 Ubiquity of Bacteria7 The Fungi: Molds and YeastsPart 3 Manipulation of Microorganisms8 Aseptic Technique9 Pure Culture TechniquesPart 4 Staining and Observations of Microorganisms10 Smear Preparation11 Simple Staining12 Negative Staining13 Capsular Staining14 Gram Staining15 Spore Staining: Two Methods16 Acid-Fast Staining: Kinyoun Method17 Motility DeterminationPart 5 Culture Methods18 Culture Media Preparation19 Enumeration of Bacteria: The Standard Plate Count20 Slide Culture: FungiPart 6 Bacterial Viruses21 Determination of a Bacteriophage Titer22 Isolation of Phage from Flies23 Phage TypingPart 7 Environmental Influences and Control of Microbial Growth24 Effects of Oxygen on Growth25 Temperature: Effects on Growth26 pH and Microbial Growth27 Water Activity and Osmotic Pressure28 Ultraviolet Light: Lethal Effects29 The Effects of Lysozyme on Bacterial Cells30 Evaluation of Alcohol: Its Effectiveness as an Antiseptic31 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method32 Evaluation of Antiseptics: The Filter Paper Disk Method33 Effectiveness of Hand ScrubbingPart 8 Identification of Unknown Bacteria34 Morphological Study of An Unknown Bacterium35 Cultural Characteristics36 Physiological Characteristics: Oxidation and Fermentation Stet Reactions37 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions38 Physiological Characteristics: Multiple Test Media39 Use of Bergey’s ManualPart 9 Miniaturized Multitest Systems40 Enterobacteriaceae Identification: The API 20E System41 Enterobacteriaceae Identification: The Enterotube II System42 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System43 Staphylococcus Identification: The API Staph SystemPart 10 Applied Microbiology44 Bacterial Food Counts45 Bacteriological Examination of Water: Most Probable Number Determination46 Bacteriological Examination of Water: The Membrane Filter Method47 Reductase Test48 Temperature: Lethal Effects49 Microbial Spoilage of Canned Food50 Microbiology of Alcohol FermentationPart 11 Medical Microbiology51 The Staphylococci: Isolation and Identification52 The Streptococci and Enterococci: Isolation and Identification53 Gram-Negative Intestinal Pathogens54 A Synthetic EpidemicPart 12 Immunology and Serology55 Slide Agglutination Test: Serological Typing56 Slide Agglutination Test for S. aureus57 Slide Agglutination Test for Streptococcus58 The Heterophile Antibody Test59 Blood GroupingAppendices