Techniques in Confocal Microscopy
Häftad, Engelska, 2010
989 kr
- Documents many diverse uses for confocal microscopy in disciplines that broadly span biology
- The methods presented include shortcuts and conveniences not included in the initial publications
- Techniques are described in a context that allows comparisons to other related methodologies
- Methodologies are laid out in a manner that stresses their general applicability and reports their potential limitations
Produktinformation
- Utgivningsdatum2010-07-23
- Mått191 x 235 x 25 mm
- Vikt880 g
- SpråkEngelska
- SerieReliable Lab Solutions
- Antal sidor544
- FörlagElsevier Science
- EAN9780123846587
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P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.
- Part I. Practical Considerations and Equipment1. Theoretical Basis of Confocal MicroscopyRobert H. Webb2. Practical Considerations in Acquiring Biological Signals from Confocal MicroscopeHao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong3. Equipment for Mass Storage and Processing of DataGuy Cox4. Antifading Agents for Confocal Fluorescence MicroscopyMiguel Berrios, Kimberly A. Conlon, and David E. Colflesh5. Mounting Techniques for Confocal MicroscopyManabu Kagayama and Yasuyuki Sasano6. Preparation of Whole Mounts and Thick Sections for Confocal MicroscopyHisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe7. Use of Confocal Microscopy to Investigate Cell Structure and FunctionGhassan Bkaily, Danielle Jacques8. Combining Laser Scanning Confocal Microscopy and Electron MicroscopyXuejun SunPart II. Functional Approaches9. Volume Measurements in Confocal MicroscopyCarlos B. Mantilla, Y.S. Prakash and Gary C. Sieck10. Quantitation of Phagocytosis by Confocal MicroscopyGeorge F. Babcock and Chad T Robinson MS11. Receptor-Ligand InternalizationGuido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti12. Quantitative Imaging of Metabolism by Two-Photon Excitation MicroscopyDavid W. Piston and Susan M. Knobel13. Trafficking of the Androgen ReceptorVirginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan14. Localization of Proteases and Peptide Receptors by Confocal MicroscopyKanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in VivoBarry R. Masters, Peter T. C. So, Ki Hean Kim, Christof Buehler, and Enrico Gratton16. Video-Rate, Scannng Slit Confocal Microscopy of Living Human Cornea in Vivo: Three-Dimensional Confocal Microscopy of the EyeBarry R. Masters and Matthias Böhnke17. In Vivo Imaging of Mammalian Central Nervous System Neurons with the In Vivo Confocal Neuroimaging (ICON) MethodSylvia Prilloff, Petra Henrich-Noack, Ralf Engelmann, Bernhard A. Sabel18. Identification of Viral Infection by Confocal MicroscopyDavid N. Howell and Sara E. Miller19. Membrane TraffickingSabine Kupzig, San San Lee, and George BantingPart III. Green Fluorescent Protein20. Monitoring of Protein Secretion with Green Fluorescent ProteinChristoph Kaether and Hans-Hermann Gerdes21. Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription ReportersXiaoning Zhao, Tommy Duong, Chiao-Chian Huang, Steven R. Kain, and Xianqiang Li22. Measuring Protein Degradation with Green Fluorescent ProteinStephen R. Cronin and Randolph Y. Hampton23. Studying Nuclear Receptors with Green Fluorescent Protein FusionsGordon L. Hager24. Signaling, Desensitization, and Trafficking of G Protein-Coupled Receptors Revealed by Green Fluorescent Protein ConjugatesLarry S. Barak, Jie Zhang, Stephen S. G. Ferguson, Stephane A. Laporte, and Marc G. Caron25. Fluorescent Proteins in Single- and Multicolor Flow CytometryLonnie Lybarger and Robert Chervenak26. Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second-Messenger Signaling in NeuronsL. A. C. Blair, K. K.Bence, and J. Marshall27. Expression of Green Fluorescent Protein and Inositol 1,4,5-Triphosphate Receptor in Xenopus laevis OocytesAtshushi Miyawaki, Julie M. Matheson, Lee G. Sayers, Akira Muto, Takayuki Michikawa, Teiichi Furuichi, and Katsuhiko Mikoshiba28. Confocal Imaging of Ca2+, pH, Electrical Potential, and Membrane Permeability in Single Living CellsJohn J. Lemasters, Donna R. Trollinger, Ting Qian, Wayne E. Cascio, and Hisayuki OhataPart IV. Laser Capture Microdissection29. Laser Capture Microdissection and Its Applications in Genomics and ProteomicsJames L. Wittliff30. Going in Vivo with Laser MicrodissectionAnette Mayer, Monika Stich, Dieter Brocksch, Karin Schütze, and Georgia Lahr31. Flourescence in Situ Hybridization of LCM-Isolated Nuclei from Paraffin SectionsDouglas J. Demetrick, Sabita K. Murthy, and Lisa M. DiFrancesco