RNA Tagging

Methods and Protocols

Häftad, Engelska, 2021

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This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease.

Produktinformation

Utgivningsdatum2021-07-26
Mått178 x 254 x 28 mm
Vikt938 g
FormatHäftad
SpråkEngelska
SerieMethods in Molecular Biology
Antal sidor490
FörlagSpringer-Verlag New York Inc.
ISBN9781071607145

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Biokemi inom Naturvetenskap och teknik

Tagging and Application of RNA Probes for Sequence-Specific Visualization of RNAs by Fluorescent In Situ Hybridization.- Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution.- Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells.- Simultaneous Detection of mRNA and Protein in S. cerevisiae by Single Molecule FISH and Immunofluorescence.- Development and Applications of Fluorogen/Light-Up RNA Aptamer Pairs for RNA Detection and More.- Visualization of Transiently Expressed mRNA in Plants Using MS2.- New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells.- In Vivo Imaging of Mobile mRNAs in Plants Using MS2.- RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi.- Utilizing Potato Virus X to Monitor RNA Movement.- A Protocol for Non-Biased Identification of RNAs Transferred between Heterologous Mammalian Cell Types Using RNA Tagging,Cell Sorting, and Sequencing.- Synthesizing Fluorescently Labeled dsRNAs and sRNAs to Visualize Fungal RNA Uptake.- Labeling of dsRNA for Fungal Uptake Detection Analysis.- Using RNA Affinity Purification Followed by Mass Spectrometry to Identify RNA-Binding Proteins (RBPs).- Plant Individual Nucleotide Resolution Crosslinking and Immunoprecipitation to Characterize RNA-Protein Complexes.- A Single-Molecule RNA Mobility Assay to Identify Proteins that Link RNAs to Molecular Motors.- Proximity-CLIP Provides a Snapshot of Protein-Occupied RNA Elements at Subcellular Resolution and Transcriptome-Wide Scale.- Double-Stranded RNA Pull-Down to Characterize Viral Replication Complexes in Plants.- CRISPR Guide RNA Design Guidelines for Efficient Genome Editing.- Live Cell CRISPR Imaging in Plant Cells with a Telomere-Specific Guide RNA.- Live-Cell Imaging of Genomic Loci Using CRISPR/Molecular Beacon Hybrid Systems.- Using RNA Tags for Multicolor Live Imaging of Chromatin Loci andTranscription in Drosophila Embryos.- Integrated Genome-Scale Analysis and Northern Blot Detection of Retrotransposon siRNAs across Plant Species.- Transfection of Small Non-Coding RNAs into Arabidopsis thaliana Protoplasts.- In Vivo Reporter System for miRNA-Mediated RNA Silencing.- TRAP-SEQ of Eukaryotic Translatomes Applied to the Detection of Polysome-Associated Long Non-Coding RNAs.- Posttranscriptional Suzuki-Miyaura Cross-Coupling Yields Labeled RNA for Conformational Analysis and Imaging.