Molecular Biology Techniques

Dr. Heather B. Miller is an Associate Professor of Biochemistry at High Point University (High Point, NC). Her area of scientific expertise is RNA biology. Her research focuses on MRSA and other pathogens’ gene expression when challenged with antibiotics and novel antibiotic adjuvants. She has mentored over 30 undergraduate students and is the Principal Investigator of a National Institutes of Health (NIH) Academic Research Enhancement Award (AREA). She was named a Henry Dreyfus Teacher-Scholar for her outstanding accomplishments in research and education in the chemical sciences. She has developed and taught multiple biochemistry and biotechnology courses and has published and pre?sented a number of peer-reviewed papers in the scholarship of teaching and learning. Dr. Miller graduated from Clarion University of Pennsylvania (Clarion, PA) with a BS in Molecular Biology/Biotechnology, and from Duke University (Durham, NC) with a PhD in Molecular Genetics and Microbiology. She completed a teaching postdoctoral position in the Biotechnology Program at North Carolina State University Dr. D. Scott Witherow is an Associate Professor of Biochemistry at The University of Tampa (Tampa, FL). His research focuses primarily on the regulation of G-protein-mediated signal transduction processes and biochemical education and pedagogy. In addition to mentoring over 20 undergraduate research students, Dr. Witherow has designed and published biochemistry lab courses and has been recognized by the American Society for Biochemistry and Molecular Biology as an education fellow for his efforts in the areas of instruction and assessment. He graduated from Rollins College (Winter Park, FL) with an AB in Chemistry and from the University of Miami (Miami, FL) with a PhD in Molecular and Cellular Pharmacology. Following traditional postdoctoral research positions at Duke University, he transitioned into pedagogy through a teaching postdoctoral position at North Carolina State University Dr. Susan Carson is a Professor of Plant and Microbial Biology at North Carolina State University (Raleigh, NC) where she has served on the faculty since 2001. She directs the Master of Microbial Biotechnology Program and also leads a uni?versity-wide faculty development program focused on enhancing students’ critical and creative thinking skills across disci?plines. Prior to her current role, Dr. Carson spent over a decade leading curriculum development for the North Carolina State Biotechnology Program and two years as a Program Officer at the National Science Foundation Division of Undergraduate Education. Her current work focuses on college-level biology education, enhancing students’ higher order thinking across disciplines, and integration of Design Thinking in a Professional Science Masters (PSM) program. She graduated from Rutgers University (New Brunswick, NJ) with a BS in Biotechnology, and from the University of North Carolina (Chapel Hill, NC) with a PhD in Microbiology.

• PART I MANIPULATION OF DNAPART II SCREENING TRANSFORMANTSPART III EXPRESSION, DETECTION, AND PURIFICATION OF RECOMBINANT PROTEINS

"Overall, this manual represents an invaluable training material on practical molecular biology for undergraduates, graduates, and inexperienced researchers. It could also introduce more experienced researchers to experiments that they have not considered previously." --Science Progress, 2012"Whilst molecular biology has been the focus of course curricula in various bioscience educational programmes, there has been a lack of well-designed laboratory manuals to recommend for the practical sessions of these courses. The third edition of ‘Molecular Biology Techniques’ is one such excellent classroom laboratory manual. It encompasses experiments for 19 laboratory sessions presented as a semester-long project that gets students involved in a comprehensive experimental story from gene cloning to protein purification. The authors have employed the versatility of the PCR technique in various experiments and have also taken advantage of the enhanced green fluorescent protein in visualising positive clones. A new section involving five laboratory sessions on measuring mRNA levels has been added to this third edition. Overall, this manual represents an invaluable training material on practical molecular biology for undergraduates, graduates, and inexperienced researchers. It could also introduce more experienced researchers to experiments that they have not considered previously." --Science Progress