Methods in Mammary Gland Biology and Breast Cancer Research

Häftad, Engelska, 2012

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This influx, which has been due primarily to the tireless efforts of breast cancer activists to gamer addi tional money from various federal and state sources, has led to a rapid expansion of research efforts by attracting numerous new investigators into the field.

Produktinformation

Utgivningsdatum2012-10-13
Mått178 x 254 x 20 mm
Vikt670 g
FormatHäftad
SpråkEngelska
Antal sidor329
FörlagSpringer-Verlag New York Inc.
ISBN9781461369271

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I. In Vivo Model Systems.- 1 Mouse Models for Mammary Cancer.- 2 Methods for the Induction of Mammary Carcinogenesis in the Rat Using either 7,12-Dimethylbenz[?]anthracene or 1-Methyl-1-Nitrosourea.- 3 A Comparison of the Salient Features of Mouse, Rat, and Human Mammary Tumorigenesis.- 4 Xenograft Models of Human Breast Cancer Lines and of the MCF10AT Model of Human Premalignant, Proliferative Breast Disease.- 5 Implantation and Characterization of Human Breast Carcinomas in SCID Mice.- II. Special Techniques for In Vivo Studies.- 6 The Cleared Mammary Fat Pad and the Transplantation of Mammary Gland Morphological Structures and Cells.- 7 Preparing Mammary Gland Whole Mounts from Mice.- 8 Slow-Release Plastic Pellets (Elvax) for Localized In Situ Treatments of Mouse and Rat Mammary Tissue.- 9 Intramammary Delivery of Hormones, Growth Factors, and Cytokines.- 10 Hormonal Stimulation of the Mouse Mammary Gland.- III. In Vitro Model Systems.- 11 Collagen Gel Method for the Primary Culture of Mouse Mammary Epithelium.- 12 Chemical-Carcinogen-Induced Transformation of Primary Cultures of Mouse Mammary Epithelial Cells Grown inside Collagen Gels.- 13 Establishment of Mouse Mammary Cell Lines.- 14 Whole Organ Culture of the Mouse Mammary Gland.- 15 Working with the Mouse Mammary End Bud.- 16 Isolation and Culture of Normal Rat Mammary Epithelial Cells.- 17 Characterization of Normal Human Breast Epithelial Cell Subpopulations Isolated by Fluorescence-Activated Cell Sorting and Their Clonogenic Growth In Vitro.- 18 Isolation and Culture of Human Breast Cancer Cells from Primary Tumors and Metastases.- IV Molecular Analysis and Gene Transfer Techniques.- 19 mRNA In Situ Hybridization in the Mammary Gland.- 20 Application of In Situ PCR to Studies of the Mammary Gland.- 21Transfection of Primary Mammary Epithelial Cells by Viral and Nonviral Methods.- 22 Direct Gene Transfer into the Mammary Epithelium In Situ Using Retroviral Vectors.- 23 Intraductal Injection into the Mouse Mammary Gland.- 24 Adenoviral and Transgenic Approaches for the Conditional Deletion of Genes from Mammary Tissue.- 25 Transplantation and Tissue Recombination Techniques to Study Mammary Gland Biology.- 26 Rescue of Mammary Epithelium of Early Lethal Phenotypes by Embryonic Mammary Gland Transplantation as Exemplified with Insulin Receptor Null Mice.