Methods in Chemical Ecology Volume 1

Kenneth F. Haynes is a Professor of Entomology in the Department of Entomology, University of Kentucky, Lexington, Kentucky.

• 1. Preparation, cleanup, and preliminary fractionation of extracts.- 1.1. Introduction.- 1.2. Contamination.- 1.3. Degradation.- 1.4. Sampling and collection of volatiles.- 1.5. General methods of extraction.- 1.6. Preliminary fractionation and cleanup.- 1.7. Acknowledgments.- 1.8. References.- 2. Liquid chromatography.- 2.1. Introduction.- 2.2. Thin-layer chromatography.- 2.3. Paper chromatography.- 2.4. Low pressure chromatography.- 2.5. High-pressure or high-performance liquid chromatography.- 2.6. Size exclusion chromatography.- 2.7. Ion-exchange chromatography.- 2.8. Argentation chromatography.- 2.9. Countercurrent chromatography.- 2.10. Acknowledgments.- 2.11. References.- 3. Analytical and preparative gas chromatography.- 3.1. Introduction.- 3.2. Mechanisms of separation and useful equations.- 3.3. GC system components.- 3.4. Retention indices.- 3.5. Micropreparative gas chromatography.- 3.6. Chromatographic troubleshooting.- 3.7. References.- 4. Mass spectrometry.- 4.1. Introduction.- 4.2. Background.- 4.3. Mass analyzers.- 4.4. Ion production and sample introduction methods.- 4.5. Mass spectral database searching.- 4.6. Information content of mass spectra.- 4.7. References.- 5. Structure elucidation by NMR.- 5.1. Introduction.- 5.2. Sample preparation considerations.- 5.3. Background.- 5.4. Example: Quinine.- 5.5.1H and13C spectra.- 5.6. DEPT.- 5.7. COSY.- 5.8. TOCSY.- 5.9. Experiments based on the nuclear Overhauser effect.- 5.10. Heteronuclear correlation experiments.- 5.11. INADEQUATE.- 5.12. Heteronuclear NMR.- 5.13. Gradient Field NMR.- 5.14. References.- 6. Infrared and ultraviolet spectroscopy techniques.- 6.1. Introduction to infrared spectroscopy.- 6.2. Coupled gas chromatography-Fourier transform infrared spectroscopy instrumentation.- 6.3. The infrared spectrum.- 6.4. Survey of vapor-phase absorptions of important functional groups.- 6.5. Ultraviolet spectroscopy.- 6.6. Information from UV spectra.- 6.7. Spectra from UV detectors used with HPLC.- 6.8. Acknowledgments.- 6.9. References.- 7. Microchemical techniques.- 7.1. Introduction.- 7.2. Derivatization and degradation methods (microreactions).- 7.3. Conclusion.- 7.4. Acknowledgments.- 7.5. References.- 8. Separation of enantiomers and determination of absolute configuration.- 8.1. Introduction.- 8.2. Determination of absolute configuration by chiroptical methods.- 8.3. Formation of diastereomeric derivatives.- 8.4. Determination of absolute configuration and enantiomeric purity by NMR methods.- 8.5. Determination of absolute configuration and enantiomeric purity by liquid chromatography with chiral stationary phases.- 8.6. Determination of absolute configuration and enantiomeric purity by gas chromatography.- 8.7. Conclusion.- 8.8. Acknowledgments.- 8.9. References.- 9. Electrophysiological methods.- 9.1. Introduction.- 9.2. Electroantennogram recording.- 9.3. Single-cell recording from olfactory receptors.- 9.4. Tip-recording from contact chemoreceptors.- 9.5. Restraints for difficult insects.- 9.6. Electronic construction.- 9.7. Acknowledgments.- 9.8. References.- Appendix: List of Names and Addresses of Suppliers.

'Overall, the book has been very meticulously prepared, and the subject dealt with in a very comprehensive manner, given the book is only 380 pages long. Readers need to have a strong background in instrumental analysis to fully appreciate the details. Nonetheless, the book is easy to read and gives a good overview. It is ideal for advanced students in chemical ecology, microanalysis, and entomology.' Journal of Environmental Quality, 28 (1999)