Methods in Chemical Ecology Volume 1

Chemical Methods

Inbunden, Engelska, 1998

AvJocelyn G. Millar,Kenneth F. Haynes,Jocelyn Millar,Kenneth Haynes

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This volume consists of chemical and biological assays applicable to all five parts of the biological world. This first volume of a two-volume set details the applicability of chemical ecological techniques to investigate problems in the interactions between and among species and points to present limitations. The book is practical, with emphasis on planning, design, and execution of experiments. It points out the advantages and limitations of various techniques, the types and amounts of information to be gained from particular techniques, as well as practical experimental details as to how to prepare and execute various bioassays and procedures. This book should be of interest to graduate students and professional ecologists interested in the natural product-based chemical interactions between plants and animals and between animals and other animals.

Produktinformation

Utgivningsdatum1998-06-30
Mått155 x 235 x 28 mm
Vikt793 g
FormatInbunden
SpråkEngelska
Antal sidor390
Upplaga1998
FörlagChapman and Hall
ISBN9780412080715

Tillhör följande kategorier

Biologi inom Naturvetenskap och teknik
Biovetenskap inom Naturvetenskap och teknik
Miljöteknik inom Naturvetenskap och teknik

1. Preparation, cleanup, and preliminary fractionation of extracts.- 1.1. Introduction.- 1.2. Contamination.- 1.3. Degradation.- 1.4. Sampling and collection of volatiles.- 1.5. General methods of extraction.- 1.6. Preliminary fractionation and cleanup.- 1.7. Acknowledgments.- 1.8. References.- 2. Liquid chromatography.- 2.1. Introduction.- 2.2. Thin-layer chromatography.- 2.3. Paper chromatography.- 2.4. Low pressure chromatography.- 2.5. High-pressure or high-performance liquid chromatography.- 2.6. Size exclusion chromatography.- 2.7. Ion-exchange chromatography.- 2.8. Argentation chromatography.- 2.9. Countercurrent chromatography.- 2.10. Acknowledgments.- 2.11. References.- 3. Analytical and preparative gas chromatography.- 3.1. Introduction.- 3.2. Mechanisms of separation and useful equations.- 3.3. GC system components.- 3.4. Retention indices.- 3.5. Micropreparative gas chromatography.- 3.6. Chromatographic troubleshooting.- 3.7. References.- 4. Mass spectrometry.- 4.1. Introduction.- 4.2. Background.- 4.3. Mass analyzers.- 4.4. Ion production and sample introduction methods.- 4.5. Mass spectral database searching.- 4.6. Information content of mass spectra.- 4.7. References.- 5. Structure elucidation by NMR.- 5.1. Introduction.- 5.2. Sample preparation considerations.- 5.3. Background.- 5.4. Example: Quinine.- 5.5.1H and13C spectra.- 5.6. DEPT.- 5.7. COSY.- 5.8. TOCSY.- 5.9. Experiments based on the nuclear Overhauser effect.- 5.10. Heteronuclear correlation experiments.- 5.11. INADEQUATE.- 5.12. Heteronuclear NMR.- 5.13. Gradient Field NMR.- 5.14. References.- 6. Infrared and ultraviolet spectroscopy techniques.- 6.1. Introduction to infrared spectroscopy.- 6.2. Coupled gas chromatography-Fourier transform infrared spectroscopy instrumentation.- 6.3. The infrared spectrum.- 6.4. Survey of vapor-phase absorptions of important functional groups.- 6.5. Ultraviolet spectroscopy.- 6.6. Information from UV spectra.- 6.7. Spectra from UV detectors used with HPLC.- 6.8. Acknowledgments.- 6.9. References.- 7. Microchemical techniques.- 7.1. Introduction.- 7.2. Derivatization and degradation methods (microreactions).- 7.3. Conclusion.- 7.4. Acknowledgments.- 7.5. References.- 8. Separation of enantiomers and determination of absolute configuration.- 8.1. Introduction.- 8.2. Determination of absolute configuration by chiroptical methods.- 8.3. Formation of diastereomeric derivatives.- 8.4. Determination of absolute configuration and enantiomeric purity by NMR methods.- 8.5. Determination of absolute configuration and enantiomeric purity by liquid chromatography with chiral stationary phases.- 8.6. Determination of absolute configuration and enantiomeric purity by gas chromatography.- 8.7. Conclusion.- 8.8. Acknowledgments.- 8.9. References.- 9. Electrophysiological methods.- 9.1. Introduction.- 9.2. Electroantennogram recording.- 9.3. Single-cell recording from olfactory receptors.- 9.4. Tip-recording from contact chemoreceptors.- 9.5. Restraints for difficult insects.- 9.6. Electronic construction.- 9.7. Acknowledgments.- 9.8. References.- Appendix: List of Names and Addresses of Suppliers.

'Overall, the book has been very meticulously prepared, and the subject dealt with in a very comprehensive manner, given the book is only 380 pages long. Readers need to have a strong background in instrumental analysis to fully appreciate the details. Nonetheless, the book is easy to read and gives a good overview. It is ideal for advanced students in chemical ecology, microanalysis, and entomology.' Journal of Environmental Quality, 28 (1999)